製品の概要

  • 製品名Anti-Hexokinase Type III antibody
    Hexokinase Type III 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Hexokinase Type III
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Hexokinase Type III .

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Human liver and spleen tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).

ターゲット情報

  • パスウェイCarbohydrate metabolism; hexose metabolism.
  • 配列類似性Belongs to the hexokinase family.
  • ドメインThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP:D hexose 6 phosphotransferase antibody
    • Hexokinase type III antibody
    • Hexokinase-3 antibody
    • Hexokinase3 antibody
    • HK III antibody
    • Hk3 antibody
    • HKIII antibody
    • HXK3 antibody
    • HXK3_HUMAN antibody
    see all

Anti-Hexokinase Type III antibody 画像

  • All lanes : Anti-Hexokinase Type III antibody (ab91097) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
    Additional bands at : 175 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab91097 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91097 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Hexokinase Type III antibody (ab91097) 使用論文

ab91097 has not yet been referenced specifically in any publications.

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