製品の概要

  • 製品名
    Anti-Hexokinase II antibody
    Hexokinase II 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Hexokinase II
  • 由来種
    Rabbit
  • 特異性
    ab78259 reacts with the Hexokinase II (102 kDa). No reactivity with the Type I or Type III isozymes is observed by immunoblot or ELISA.
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Rat, Hamster, Human
    交差が予測される動物種: Mammal
  • 免疫原

    Recombinant full length Hexokinase II protein (Rat)

  • ポジティブ・コントロール
    • Insulin-responsive tissues such as skeletal muscle.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/10000. Predicted molecular weight: 102 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 組織特異性
    Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
  • パスウェイ
    Carbohydrate metabolism; hexose metabolism.
  • 配列類似性
    Belongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • ドメイン
    The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • 細胞内局在
    Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686M1669 antibody
    • Hexokinase 2 antibody
    • Hexokinase 2 muscle antibody
    • Hexokinase type II antibody
    • Hexokinase-2 antibody
    • HK 2 antibody
    • HK II antibody
    • HK2 antibody
    • HKII antibody
    • HxK 2 antibody
    • HxK2 antibody
    • HXK2_HUMAN antibody
    • Muscle form hexokinase antibody
    see all

画像

  • The Type I isozyme (purified from rat brain) and the rat Type II and Type III isozymes (expressed in yeast) were used for SDS gel electrophoresis and immunoblotting. The top panel shows the gel after staining with Coomassie Blue (5 µg protein in each lane). The bottom three panels show replicate immunoblots probed with a monoclonal to Hexokinase I, ab78259 or a monoclonal to Hexokinase III.

参考文献

This product has been referenced in:
  • Waskova-Arnostova P  et al. Chronic hypoxia enhances expression and activity of mitochondrial creatine kinase and hexokinase in the rat ventricular myocardium. Cell Physiol Biochem 33:310-20 (2014). Read more (PubMed: 24525799) »

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab78259.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録