製品の概要

  • 製品名Anti-Hexokinase II antibody
    Hexokinase II 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Hexokinase II
  • 特異性This antibody is likely to cross-react with Hexokinase I (93% identity with the immunogen).
  • アプリケーション適用あり: WB, ELISA, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum
  • 免疫原

    Recombinant fragment: IVKEVCTVVA RRAAQLCGAG MAAVVDRIRE NRGLDALKVT VGVDGTLYKL HPHFAKVMHE TVKDLAPKCD VSFLQSEDGS GKGAALITAV ACRIREAGQR , corresponding to amino acids 818-917 of Human Hexokinase IIwith a proprietary tag (MW of the tag alone is 26 KDa).

  • ポジティブ・コントロール
    • A431 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 102 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 組織特異性Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
  • パスウェイCarbohydrate metabolism; hexose metabolism.
  • 配列類似性Belongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • ドメインThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • 細胞内局在Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686M1669 antibody
    • Hexokinase 2 antibody
    • Hexokinase 2 muscle antibody
    • Hexokinase type II antibody
    • Hexokinase-2 antibody
    • HK 2 antibody
    • HK II antibody
    • HK2 antibody
    • HKII antibody
    • HxK 2 antibody
    • HxK2 antibody
    • HXK2_HUMAN antibody
    • Muscle form hexokinase antibody
    see all

Anti-Hexokinase II antibody 画像

  • Anti-Hexokinase II antibody (ab76959) at 1/1000 dilution + A-431 cell lysate at 25 µg

    Predicted band size : 102 kDa
  • Anti-Hexokinase II antibody (ab76959) at 1/2500 dilution + Immunizing peptide at 0.1 µg

    Predicted band size : 102 kDa
  • Detection limit for recombinant GST tagged HK2 is approximately 0.3ng/ml as a capture antibody.
  • Overlay histogram showing K562 cells stained with ab76959 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76959, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Hexokinase II antibody (ab76959) 使用論文

This product has been referenced in:
  • Yao M  et al. Dicer mediating the expression of miR-143 and miR-155 regulates hexokinase II associated cellular response to hypoxia. Am J Physiol Lung Cell Mol Physiol 307:L829-37 (2014). Read more (PubMed: 25172909) »
  • de Weille J  et al. Similar pyruvate kinase modifications in glioblastoma cells by 7ß-hydroxycholesterol and glutamine withdrawal. Biochem Pharmacol 86:161-7 (2013). Read more (PubMed: 23537700) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (13%)
Sample Human Cell lysate - whole cell (HCT116, MiaPaca2 and RKO)
Specification HCT116, MiaPaca2 and RKO
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Mr. Christian Marx

Verified customer

投稿 Aug 27 2013

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacementcontaining 1 vialeach of ab38237,13533,38295.

To check the status of the or...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"