The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/60 - 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa).
組織特異性Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
パスウェイCarbohydrate metabolism; hexose metabolism.
配列類似性Belongs to the hexokinase family. Contains 2 hexokinase domains.
ドメインThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
細胞内局在Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.