製品の概要

  • 製品名Anti-Hexokinase II antibody [3D3]
    Hexokinase II 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3D3] to Hexokinase II
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to Human Hexokinase II.

  • ポジティブ・コントロール
    • Jurkat, Hela and HEK293 cell lysates; Human esophagus and lung cancer tissues; K562 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 102 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.

ターゲット情報

  • 組織特異性Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.
  • パスウェイCarbohydrate metabolism; hexose metabolism.
  • 配列類似性Belongs to the hexokinase family.
    Contains 2 hexokinase domains.
  • ドメインThe N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule.
  • 細胞内局在Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686M1669 antibody
    • Hexokinase 2 antibody
    • Hexokinase 2 muscle antibody
    • Hexokinase type II antibody
    • Hexokinase-2 antibody
    • HK 2 antibody
    • HK II antibody
    • HK2 antibody
    • HKII antibody
    • HxK 2 antibody
    • HxK2 antibody
    • HXK2_HUMAN antibody
    • Muscle form hexokinase antibody
    see all

Anti-Hexokinase II antibody [3D3] 画像

  • All lanes : Anti-Hexokinase II antibody [3D3] (ab104836) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HEK293 cell lysate


    Predicted band size : 102 kDa
  • ab104836 at 1/200 dilution staining Hexokinase II in Human esophagus cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
  • ab104836 at 1/200 dilution staining Hexokinase II in Human lung cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
  • ab104836 staining Hexokinase II in K562 cells by Immunofluorescence (green) and negative control (purple).
  • Immunohistochemical analysis of Human skin tissue, staining Hexokinase II with ab104836.

    Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/250 in 5% NGS + 10% BSA in PBS) for 16 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/250) was used as the secondary antibody.

    See Abreview

Anti-Hexokinase II antibody [3D3] (ab104836) 使用論文

This product has been referenced in:
  • Pertega-Gomes N  et al. A glycolytic phenotype is associated with prostate cancer progression and aggressiveness: a role for monocarboxylate transporters as metabolic targets for therapy. J Pathol 236:517-30 (2015). IHC ; Human . Read more (PubMed: 25875424) »
  • Yao M  et al. Dicer mediating the expression of miR-143 and miR-155 regulates hexokinase II associated cellular response to hypoxia. Am J Physiol Lung Cell Mol Physiol 307:L829-37 (2014). Read more (PubMed: 25172909) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse prostate (Pten knockout))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
Permeabilization No
Specification Mouse prostate (Pten knockout)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Dr. Lucy Penfold

Verified customer

投稿 Nov 09 2016

Application Western blot
Sample Baboon Tissue lysate - whole (fetal skeletal muscle)
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Loading amount 25 µg
Treatment 115 ug/kg body weight/day letrozole in utero for 100 days
Specification fetal skeletal muscle
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Soon-ok Kim

Verified customer

投稿 Aug 19 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (MRC5 normal lung fibroblasts)
Specification MRC5 normal lung fibroblasts
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

投稿 Feb 27 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 100mM sodium Cytrat
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Hadas Pahima

Verified customer

投稿 Oct 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 100mM sodium citrate
Permeabilization No
Blocking step (agent) for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

ilan sela

Verified customer

投稿 May 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 30 µg
Specification HeLa
Treatment CoCl2 for 24h
Gel Running Conditions Reduced Denaturing (12.5)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Ms. Hadas Pahima

Verified customer

投稿 Apr 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma neurosphere cells (primary))
Loading amount 30 µg
Specification Glioblastoma neurosphere cells (primary)
Gel Running Conditions Reduced Denaturing (12.5% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Dec 06 2011

Thank you for your inquiry. We did not test for cross-reactivity with hexokinase I. Please contact us with any other questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"