Hexokinase 1 overexpression 293T lysate (whole cell) (ab94049)

製品の概要

  • 製品名Hexokinase 1 overexpression 293T lysate (whole cell)
  • 製品の詳細
    Hexokinase 1 overexpression lysate
  • 特記事項ab94049 is a 293T cell transfected lysate in which Human Hexokinase 1 has been transiently over-expressed using a pCMV-Hexokinase 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景Disease: Hexokinase deficiency Neuropathy, hereditary motor and sensory, Russe type Domain: The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule. Pathway: Carbohydrate metabolism; hexose metabolism. Similarity: Belongs to the hexokinase family. Contains 2 hexokinase domains. Tissue specificity: Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.

アプリケーション

Our Abpromise guarantee covers the use of ab94049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

Hexokinase 1 overexpression 293T lysate (whole cell) 画像

  • ab94049 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Hexokinase 1 antibody (ab55144) at 1/500 dilution

    Lane 1 : Hexokinase 1 overexpression 293T lysate (whole cell) (ab94049)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Hexokinase 1 overexpression 293T lysate (whole cell) (ab94049) 使用論文

ab94049 has not yet been referenced specifically in any publications.

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