製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HEXB
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from the C-terminal of human HEXB.

  • ポジティブ・コントロール
    • Colon carcinoma tissue. Jurkat cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
ELISA
  • 追加情報
    ELISA: 1/20000.
    IHC-P: 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method, boiling for 2-3 min, before commencing with IHC staining protocol.
    WB: 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues.
    • 関連疾患
      Defects in HEXB are the cause of GM2-gangliosidosis type 2 (GM2G2) [MIM:268800]; also known as Sandhoff disease. GM2-gangliosidosis is an autosomal recessive lysosomal storage disease marked by the accumulation of GM2 gangliosides in the neuronal cells. GM2G2 is clinically indistinguishable from GM2-gangliosidosis type 1, presenting startle reactions, early blindness, progressive motor and mental deterioration, macrocephaly and cherry-red spots on the macula.
    • 配列類似性
      Belongs to the glycosyl hydrolase 20 family.
    • 翻訳後修飾
      N-linked glycans at Asn-142 and Asn-190 consist of Man(3)-GlcNAc(2) and Man(5 to 7)-GlcNAc(2), respectively.
      The beta-A and beta-B chains are produced by proteolytic processing of the precursor beta chain.
    • 細胞内局在
      Lysosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Beta hexosaminidase beta chain antibody
      • Beta hexosaminidase subunit beta antibody
      • Beta N acetylhexosaminidase antibody
      • Beta-hexosaminidase subunit beta chain A antibody
      • Beta-N-acetylhexosaminidase subunit beta antibody
      • Cervical cancer proto oncogene 7 protein antibody
      • Cervical cancer proto-oncogene 7 protein antibody
      • ENC 1AS antibody
      • Epididymis luminal protein 248 antibody
      • HCC 7 antibody
      • HCC-7 antibody
      • HCC7 antibody
      • HEL 248 antibody
      • HEX B antibody
      • Hexb antibody
      • HEXB_HUMAN antibody
      • Hexosaminidase B (beta polypeptide) antibody
      • Hexosaminidase B antibody
      • Hexosaminidase subunit B antibody
      • HexosaminidaseB antibody
      • N acetyl beta glucosaminidase antibody
      • N-acetyl-beta-glucosaminidase subunit beta antibody
      see all

    画像

    • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using HEXB antibody (ab65001) at 1/50 dilution, in the presence (right panel) and absence (left panel) of immunising peptide. Antigen retrieval was performed by boiling in a pressure cooker for 2-3 min. A Polymer system was used for signal enhancing. Stained using DAB.
    • All lanes : Anti-HEXB antibody (ab65001) at 1/500 dilution

      Lane 1 : Extracts from Jurkat cells
      Lane 2 : Extracts from Jurkat cells with immunizing peptide at 5 µg

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 63 kDa
      Observed band size : 63 kDa

    参考文献

    ab65001 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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