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RabMAb

Anti-Hex 抗体 [EPR3361] (ab79392)

製品の概要

  • 製品名
    Anti-Hex antibody [EPR3361]
    Hex 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3361] to Hex
  • アプリケーション
    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Hex aa 100-200.

  • ポジティブ・コントロール
    • WB: MCF7, T47-D, K562, HL-60, HepG2 and A549 cell lysates. ICC/IF: HepG2 cells. Flow Cyt: A549 cells. IP: MCF7 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IP 1/30.

For unpurified use at 1/40.

  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • ターゲット情報

    • 機能
      Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.
    • 組織特異性
      Liver and promyelocytic leukemia cell line HL60.
    • 配列類似性
      Contains 1 homeobox DNA-binding domain.
    • 発生段階
      Expressed during hematopoiesis.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Hematopoietically expressed homeobox antibody
      • Hematopoietically-expressed homeobox protein HHEX antibody
      • HEX antibody
      • HHEX antibody
      • HHEX_HUMAN antibody
      • HMPH antibody
      • Homeobox hematopoietically expressed antibody
      • Homeobox protein HEX antibody
      • Homeobox protein PRH antibody
      • HOX11L PEN antibody
      • PRH antibody
      • PRHX antibody
      • Proline rich homeodomain containing transcription factor antibody
      see all

    Anti-Hex antibody [EPR3361] 画像

    • All lanes : Anti-Hex antibody [EPR3361] (ab79392) at 1/2000 dilution (purified)

      Lane 1 : MCF-7 cell lysate
      Lane 2 : T47-D cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : HL-60 cell lysate
      Lane 5 : HepG2 cell lysate
      Lane 6 : A549 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size : 30 kDa
      Observed band size : 30 kDa

      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-Hex antibody [EPR3361] (ab79392) at 1/2000 dilution (unpurified)

      Lane 1 : MCF7 cell lysate
      Lane 2 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 30 kDa
      Observed band size : 30 kDa
    • Purified ab79392 (1/30) immunoprecipitating Hex in MCF-7 whole cell lysate (Lane +). For western blotting an Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG (1/500) was used as the secondary antibody.

      Lane (-): Rabbit monoclonal IgG (ab172730) instead of ab79392 in MCF-7 whole cell lysate.

      Blocking and diluting buffer: 5% NFDM/TBST.

    Anti-Hex antibody [EPR3361] (ab79392) 使用論文

    This product has been referenced in:
    • Zhao J  et al. Expression analyses of the genes harbored by the type 2 diabetes and pediatric BMI associated locus on 10q23. BMC Med Genet 13:89 (2012). WB . Read more (PubMed: 22998375) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab79392.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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