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Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Hex.
(Peptide available as ab28465.)
Our Abpromise guarantee covers the use of ab34222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa).Can be blocked with Human Hex peptide (ab28465).|
ab34222 staining Hex in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/100 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor®488 conjugated goat polyclonal to rabbit IgG at 1/1000 dilution, was used as secondary.