Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) 抗体 (ab50966)

製品の概要

  • 製品名
    Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody
    Heterogeneous Nuclear Ribonucleoprotein (A1-like) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Heterogeneous Nuclear Ribonucleoprotein (A1-like)
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Chimpanzee
  • 免疫原

    A region within synthetic peptide: MSKSASPKEP EQLRKLFIGG LSFETTDESL RSHFEQWGTL TDCVVMRDPN, corresponding to N terminal amino acids 1-50 of Human Heterogeneous Nuclear Ribonucleoprotein (A1-like)

  • ポジティブ・コントロール
    • WB: Jurkat cell lysate. IHC-P: Human stomach tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

画像

  • All lanes : Anti-Heterogeneous Nuclear Ribonucleoprotein (A1-like) antibody (ab50966) at 1.25 µg/ml

    Lane 1 : MW marker
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • ab50966, at a concentration of 8µg/ml, staining Heterogeneous Nuclear Ribonucleoprotein (A1-like) in paraffin embedded human stomach tissue sections. The positive cells (indicated by arrows) are epithelial cells of fundic glands.

参考文献

ab50966 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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