製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB 1/200 - 1/1000. Predicted molecular weight: 19 kDa.
IHC-Fr 1/200 - 1/1000.

This product has not been tested with paraffin sections.

ターゲット情報

  • 機能
    Transcriptional repressor of genes that require a bHLH protein for their transcription.
  • 組織特異性
    Expressed in fetal heart and brain tumors.
  • 配列類似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 Orange domain.
  • ドメイン
    Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
    The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bHLHb38 antibody
    • Class B basic helix-loop-helix protein 38 antibody
    • Hairy and enhancer of split 5 antibody
    • Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody
    • HES 5 antibody
    • hes family bHLH transcription factor 5 antibody
    • Hes5 antibody
    • HES5 protein antibody
    • HES5_HUMAN antibody
    • MGI:104876 antibody
    • Transcription factor HES-5 antibody
    see all

Anti-HES5 antibody 画像

  • ab25374 staining the cortex of e14.5 mouse embryo by IHC-Fr.  The tissue was frozen and cut into 10 µm slices on a cyrostat then fixed and blocked prior to incubating with the primary antibody (diluted 1/100) for 90 minutes.  A fluorescent conjugated secondary antibody was incubated with the sample for 30 minutes.  HES-5 is shown in red in the nuclear regions; the blue staining is Hoechst nuclear counter stain.

Anti-HES5 antibody (ab25374) 使用論文

This product has been referenced in:
  • Marathe S  et al. Jagged1 Is Altered in Alzheimer's Disease and Regulates Spatial Memory Processing. Front Cell Neurosci 11:220 (2017). IHC . Read more (PubMed: 28848392) »
  • Pan Z  et al. The Expression of Markers Related to Ovarian Germline Stem Cells in the Mouse Ovarian Surface Epithelium and the Correlation with Notch Signaling Pathway. Cell Physiol Biochem 37:2311-22 (2015). Read more (PubMed: 26645720) »

See all 9 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (differentiated induced pluripotent stem cells)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
differentiated induced pluripotent stem cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 19 2017

Thank you for your inquiry.

I am happy to confirm that ab25374 is tested and guaranteed for WB with mouse and human samples.

It was tested using standard WB procedure which can be found here:

http://www.abcam.com/index.htm...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse brain)
Specification
mouse brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 26 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Neural cells)
Specification
Neural cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Feb 24 2010

The Uniprot localization of Hes5 is listed as Nuclear (by similarity) to other transcriptional repressors. However, Hes5 is widely expressed in the developing nervous system (Akazawa et al., 1992) and in embryos Hes5 is ubiquitously expressed inside...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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