製品の概要

  • 製品名
    Anti-HES4 antibody
  • 製品の詳細
    Rabbit polyclonal to HES4
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chicken, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide: AADTPGKPSA SPMAGAPASA SRTPDKPRSA AEHRKSSKPV MEKRRRARIN , corresponding to amino acids 2-51 of Human HES4

  • ポジティブ・コントロール
    • Transfected 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

ターゲット情報

  • 関連性
    HES4 may be a transcriptional repressor. It is expressed in stomach, pancreas, ovary, uterus, eye and bone. It has an N-terminal bHLH domain.
  • 細胞内局在
    Nuclear
  • 参照データベース
  • 別名
    • bHLH factor Hes4 antibody
    • Hairy and enhancer of split 4 antibody
    • Transcription factor HES 4 antibody

画像

  • Anti-HES4 antibody (ab66377) at 2 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 24 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab66377 has not yet been referenced specifically in any publications.

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