製品の概要

  • 製品名Anti-Hes1 antibody [EPR4226]
    Hes1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4226] to Hes1
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Hes1 aa 200-300.

  • ポジティブ・コントロール
    • Fetal brain, PC12, and SH-SY5Y cell lysates. Human placenta tissue.
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/40 - 1/100.

 

WB 1/500 - 1/1000. Predicted molecular weight: 30 kDa.

 

IHC-P 1/90 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Flow Cyt 1/20 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
    • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 Orange domain.
    • ドメインHas a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
      The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
      The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • bHLHb39 antibody
      • C-HAIRY1 antibody
      • c-hairy1A antibody
      • Class B basic helix-loop-helix protein 39 antibody
      • FLJ20408 antibody
      • Hairy and enhancer of split 1 (Drosophila) antibody
      • Hairy and enhancer of split 1 antibody
      • Hairy homolog (Drosophila) antibody
      • Hairy homolog antibody
      • Hairy like antibody
      • Hairy, Drosophila, homolog of antibody
      • Hairy-like protein antibody
      • Hairy/enhancer of split, Drosophila, homolog of, 1 antibody
      • HAIRY1 antibody
      • HES-1 antibody
      • hes1 antibody
      • Hes1 hairy and enhancer of split 1 (Drosophila) antibody
      • HES1_HUMAN antibody
      • HHL antibody
      • HL antibody
      • HRY antibody
      • MGC129109 antibody
      • OTTHUMP00000209031 antibody
      • RHL antibody
      • Transcription factor HES-1 antibody
      see all

    Anti-Hes1 antibody [EPR4226] 画像

    • Anti-Hes1 antibody [EPR4226] (ab108937) at 1/1000 dilution (purified) + SH-SY5Y cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 30 kDa
      Observed band size : 30 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Primary ab concentration dilution: 1:200, (0.5ug/ml), Secondary ab: ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG, Secondary ab concentration: Prediluted, Tissue: Human brain, Fixative: Paraffin-embedded sections, Counter stain: Hematoxylin Antigen retrieval: Perform heat mediated antigen retrieval using Tris/EDTA Buffer, PH9

    • Immunofluorescent staining of SH-SY5Y cells (fixed with 4% PFA and permeablized with TritonX 100) with purified ab108937 at a dilution of 1/100. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

    • Overlay histogram showing SH-SY5Y cells stained with unpurified ab108937 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108937, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • All lanes : Anti-Hes1 antibody [EPR4226] (ab108937) at 1/1000 dilution (unpurified)

      Lane 1 : Fetal brain lysate
      Lane 2 : PC12 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 30 kDa
    • Immunohistochemical staining of Hes1 in paraffin-embedded human placenta tissue with unpurified ab108937 at 1/250 dilution.

    • Immunohistochemical staining of paraffin embedded human brain with unpurified ab108937 at a working dilution of 1 in 90. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    • Immunofluorescent staining of SH-SY5Y cells (fixed with 4% PFA and permeablized with TritonX 100) with unpurified ab108937 at a dilution of 1/40. An Alexa Fluor® 555 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

    Anti-Hes1 antibody [EPR4226] (ab108937) 使用論文

    This product has been referenced in:
    • Yao J  et al. Interference of Notch1 inhibits the growth of glioma cancer cells by inducing cell autophagy and down-regulation of Notch1-Hes-1 signaling pathway. Med Oncol 32:610 (2015). WB ; Human . Read more (PubMed: 25920606) »
    • Xie F  et al. Vaccarin attenuates the human EA.hy926 endothelial cell oxidative stress injury through inhibition of Notch signaling. Int J Mol Med 35:135-42 (2015). Human . Read more (PubMed: 25352009) »

    See all 5 Publications for this product

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    I am sorry to hear that the antibody is not working as expected.

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    Thank you for your enquiry.

    I am sorry but it is not known which specific cell type is being stained in the placental tissue.

    Please contact us again if you have any further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"