Anti-Hes1 抗体 [EPR4226] (ab108937)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4226] to Hes1
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Hes1 antibody [EPR4226]
Hes1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4226] to Hes1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details
適用なし: IP -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: SH-SY5Y, MOLT-4, MDA-MB-231, MCF7, K-562, HUVEC, HepG2, Human brain, Human liver, Human placenta.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4226 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108937の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (2) |
1/1000.
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WB | (4) |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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IHC-P | (5) |
1/90 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/20 - 1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
1/1000. |
WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
IHC-P
1/90 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/20 - 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
ドメイン
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3280 Human
- Entrez Gene: 15205 Mouse
- Entrez Gene: 29577 Rat
- Omim: 139605 Human
- SwissProt: Q14469 Human
- SwissProt: P35428 Mouse
- SwissProt: Q04666 Rat
- Unigene: 250666 Human
see all -
別名
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
画像
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Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% TritonX-100 permeabilised SH-SY5Y (Human neuroblastoma epithelial cell) labelling Hes1 with ab108937 at 1/1000 dilution, followed by AlexaFluor® 488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in SH-SY5Y cell line is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain alpha Tubulin at 1/200 dilution (red). The Nuclear counterstain was DAPI (blue).
Secondary antibody only control: Secondary antibody is AlexaFluor® 488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
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All lanes : Anti-Hes1 antibody [EPR4226] (ab108937) at 1/1000 dilution
Lane 1 : SH-SY5Y (Human bone marrow epithelial) whole cell lysate
Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 6 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
Lane 7 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 8 : Human brain tissue lysate
Lane 9 : Human liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 120 secondsBlocking and diluting buffer and concentration: 5% NFDM /TBST.
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Primary ab concentration dilution: 1:200, (0.5ug/ml)
Secondary ab: ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG
Secondary ab concentration: Prediluted
Tissue: Human brain
Fixative: Paraffin-embedded sections
Counter stain: Hematoxylin
Antigen retrieval: Perform heat mediated antigen retrieval using Tris/EDTA Buffer, PH9
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Overlay histogram showing SH-SY5Y cells stained with unpurifiedab108937 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108937, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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Immunohistochemical staining of Hes1 in paraffin-embedded human placenta tissue with unpurified ab108937 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical staining of paraffin embedded human brain with unpurified ab108937 at a working dilution of 1 in 90. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (70)
ab108937 は 70 報の論文で使用されています。
- Dang L et al. Downregulation of sperm-associated antigen 5 inhibits melanoma progression by regulating forkhead box protein M1/A disintegrin and metalloproteinase 17/NOTCH1 signaling. Bioengineered 13:4744-4756 (2022). PubMed: 35138218
- Cai W et al. The Notch pathway attenuates burn-induced acute lung injury in rats by repressing reactive oxygen species. Burns Trauma 10:tkac008 (2022). PubMed: 35441079
- Tang Y & Cao Y Curcumin Inhibits the Growth and Metastasis of Melanoma via miR-222-3p/SOX10/Notch Axis. Dis Markers 2022:3129781 (2022). PubMed: 35585935
- Chen Y et al. Galectin-3 enhances trastuzumab resistance by regulating cancer malignancy and stemness in HER2-positive breast cancer cells. Thorac Cancer 13:1961-1973 (2022). PubMed: 35599381
- Yang G et al. Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway. Int J Mol Sci 23:N/A (2022). PubMed: 35682694