Anti-Hes1 抗体 [OTI2D2] (ab119776)
Key features and details
- Mouse monoclonal [OTI2D2] to Hes1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Hes1 antibody [OTI2D2]
Hes1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OTI2D2] to Hes1 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant full length Human Hes1 produced in HEK293T cells (NP_005515).
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ポジティブ・コントロール
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY Hes1 cDNA. ICC/IF: MCF7 cell lysates; COS7 cells transiently transfected by pCMV6-ENTRY Hes1 cDNA. Flow Cyt (Intra): Jurkat cells. IHC-P: Human thyroid, thyroid carcinoma and bladder carcinoma tissues.
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特記事項
Dilute in PBS (pH7.3) before use.
This product was changed from ascites to tissue culture supernatant on the 4th of June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
OTI2D2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab119776の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF | (2) |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain. -
ドメイン
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3280 Human
- Omim: 139605 Human
- SwissProt: Q14469 Human
- Unigene: 250666 Human
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別名
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
画像
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All lanes : Anti-Hes1 antibody [OTI2D2] (ab119776) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Hes1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 30 kDaHEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211709)
This image was generated using the ascites version of the product.
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ab119776, at 1/150 dilution, staining Hes1 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
This image was generated using the ascites version of the product.
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ab119776, at 1/150 dilution, staining Hes1 in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
This image was generated using the ascites version of the product.
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ab119776, at 1/150 dilution, staining Hes1 in paraffin-embedded Human thyroid tissue by Immunohistochemistry.
This image was generated using the ascites version of the product.
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ab119776, at 1/100 dilution, staining Hes1 in COS7 cells transiently transfected with pCMV6-ENTRY Hes1 cDNA by Immunofluorescence.
This image was generated using the ascites version of the product.
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ab119776, at 1/100 dilution, staining Hes1 (red) in Jurkat cells, compared to a nonspecific negative control antibody,(blue) by Flow cytometry (Intracellular).
This image was generated using the ascites version of the product.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab119776 は 13 報の論文で使用されています。
- Chesnais F et al. High-content image analysis to study phenotypic heterogeneity in endothelial cell monolayers. J Cell Sci 135:N/A (2022). PubMed: 34982151
- Zheng Y et al. Nogo-B promotes angiogenesis and improves cardiac repair after myocardial infarction via activating Notch1 signaling. Cell Death Dis 13:306 (2022). PubMed: 35383153
- Liu CF et al. Whole-Transcriptome Profiling of Human Heart Tissues Reveals the Potential Novel Players and Regulatory Networks in Different Cardiomyopathy Subtypes of Heart Failure. Circ Genom Precis Med 14:e003142 (2021). PubMed: 33517678
- Tao ZS et al. Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats. J Mater Sci Mater Med 33:4 (2021). PubMed: 34940936
- Fan J et al. Targeting the Notch and TGF-ß signaling pathways to prevent retinal fibrosis in vitro and in vivo. Theranostics 10:7956-7973 (2020). PubMed: 32724452