Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.
Expressed in brain and testis and detected in heart and placenta.
All lanes : Anti-HERC4 antibody (ab85732) at 0.1 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg Lane 2 : Whole cell
lysate from HeLa cells at 15 µg Lane 3 : Whole cell
lysate from HeLa cells at 5 µg Lane 4 : Whole cell
lysate from 293T cells at 50 µg Lane 5 : Whole cell
lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique
Predicted band size : 119 kDa Observed band size : 119 kDa
Exposure time : 30 seconds
Immunoprecipitation - HERC4 antibody (ab85732)
Detection of Human HERC4 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85732 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.