製品の概要

  • 製品名Anti-HERC4 antibody
    HERC4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HERC4
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1007 and 1057 of Human HERC4 (NP_071362.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 119 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.
  • 組織特異性Expressed in brain and testis and detected in heart and placenta.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 7 RCC1 repeats.
  • 発生段階Expressed in fetal brain.
  • 細胞内局在Cytoplasm > cytosol. shows a punctate cytoplasmic distribution.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZP564G092 antibody
    • HECT and RLD domain containing E3 ubiquitin protein ligase 4 antibody
    • HECT domain and RCC1 like domain containing protein 4 antibody
    • HECT domain and RCC1-like domain-containing protein 4 antibody
    • Hect domain and RLD 4 antibody
    • HERC 4 antibody
    • HERC4 antibody
    • HERC4_HUMAN antibody
    • KIAA1593 antibody
    • Probable E3 ubiquitin protein ligase HERC4 antibody
    • Probable E3 ubiquitin-protein ligase HERC4 antibody
    see all

Anti-HERC4 antibody 画像

  • All lanes : Anti-HERC4 antibody (ab85732) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 119 kDa
    Observed band size : 119 kDa


    Exposure time : 30 seconds
  • Detection of Human HERC4 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85732 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-HERC4 antibody (ab85732) 使用論文

ab85732 has not yet been referenced specifically in any publications.

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