Anti-HERC2 抗体 (ab85832)
Key features and details
- Rabbit polyclonal to HERC2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-HERC2 antibody
HERC2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to HERC2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to Human HERC2 aa 4750-4850.
Database link: O95714 -
ポジティブ・コントロール
- Whole cell lysate from HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85832の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 527 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 527 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity. -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Mental retardation, autosomal recessive 38 -
配列類似性
Contains 1 cytochrome b5 heme-binding domain.
Contains 1 DOC domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 MIB/HERC2 domain.
Contains 21 RCC1 repeats.
Contains 1 ZZ-type zinc finger. -
ドメイン
The ZZ-type zinc finger mediates binding to SUMO1, and at lowe level SUMO2.
The RCC1 repeats are grouped into three seven-bladed beta-propeller regions. -
翻訳後修飾
Phosphorylation at Thr-4827 is required for interaction with RNF8.
Sumoylated with SUMO1 by PIAS4 in response to double-strand breaks (DSBs), promoting the interaction with RNF8. -
細胞内局在
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Nucleus. Recruited to sites of DNA damage in response to ionizing radiation (IR) via its interaction with RNF8. May loose association with centrosomes during mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 8924 Human
- Entrez Gene: 15204 Mouse
- Omim: 605837 Human
- SwissProt: O95714 Human
- SwissProt: Q4U2R1 Mouse
- Unigene: 434890 Human
- Unigene: 610412 Human
- Unigene: 741019 Human
see all -
別名
- D15F37S1 antibody
- E3 ubiquitin-protein ligase HERC2 antibody
- HECT and RLD domain containing E3 ubiquitin protein ligase 2 antibody
see all
画像
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All lanes : Anti-HERC2 antibody (ab85832) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.
Predicted band size: 527 kDa
Observed band size: 527 kDa
Additional bands at: 160 kDa, 260 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Immunoprecipitation/ Western Blot
For IP: Lane 1: ab85832 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab85832 at 1µg/ml for WBWhole cell lysate from HeLa cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab85832 は 6 報の論文で使用されています。
- Wang LQ et al. LncRNA-Fendrr protects against the ubiquitination and degradation of NLRC4 protein through HERC2 to regulate the pyroptosis of microglia. Mol Med 27:39 (2021). PubMed: 33858325
- Xu S et al. ASPM promotes homologous recombination-mediated DNA repair by safeguarding BRCA1 stability. iScience 24:102534 (2021). PubMed: 34142045
- Harris LD et al. The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1. J Biol Chem 296:100246 (2021). PubMed: 33853758
- Liu J et al. Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2. STAR Protoc 1:100155 (2020). PubMed: 33377049
- Saez I et al. Insights into the ubiquitin-proteasome system of human embryonic stem cells. Sci Rep 8:4092 (2018). PubMed: 29511261
- Moroishi T et al. HERC2 targets the iron regulator FBXL5 for degradation and modulates iron metabolism. J Biol Chem 289:16430-41 (2014). PubMed: 24778179