製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Hepsin
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Cow, Dog, Macaque monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Hepsin.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HepG2 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Plays an essential role in cell growth and maintenance of cell morphology.
  • 組織特異性
    Present in most tissues, with the highest level in liver.
  • 配列類似性
    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 1 SRCR domain.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 3.4.21. antibody
    • HEPS antibody
    • HEPS_HUMAN antibody
    • Hepsin antibody
    • Hpn antibody
    • Serine protease hepsin antibody
    • Serine protease hepsin catalytic chain antibody
    • Serine protease hepsin non catalytic chain antibody
    • TMPRSS 1 antibody
    • TMPRSS1 antibody
    • Transmembrane protease serine 1 antibody
    see all

画像

  • Anti-Hepsin antibody (ab73133) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)


    Exposure time : 16 minutes
  • ab73133 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab73133 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde (4%, 10min) fixed HeLa, Hek293, and HepG2 cell types at 5ug/ml.

参考文献

ab73133 has not yet been referenced specifically in any publications.

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