Anti-Hepsin/HPN 抗体 (ab73133)
Key features and details
- Rabbit polyclonal to Hepsin/HPN
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Hepsin/HPN antibody
Hepsin/HPN 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hepsin/HPN -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Cow, Dog, Macaque monkey, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Recombinant Human Hepsin/HPN protein (ab114907) can be used as a positive control in WB. This antibody gave a positive signal in HepG2 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73133の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Plays an essential role in cell growth and maintenance of cell morphology. -
組織特異性
Present in most tissues, with the highest level in liver. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 508148 Cow
- Entrez Gene: 3249 Human
- Entrez Gene: 100173788 Orangutan
- Omim: 142440 Human
- SwissProt: P05981 Human
- SwissProt: Q5R5E8 Orangutan
- Unigene: 182385 Human
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別名
- EC 3.4.21. antibody
- HEPS antibody
- HEPS_HUMAN antibody
see all
画像
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Anti-Hepsin/HPN antibody (ab73133) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesHepsin/HPN contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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ab73133 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab73133 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde (4%, 10min) fixed HeLa, Hek293, and HepG2 cell types at 5ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab73133 は 1 報の論文で使用されています。
- Ma X et al. Identification of a distinct luminal subgroup diagnosing and stratifying early stage prostate cancer by tissue-based single-cell RNA sequencing. Mol Cancer 19:147 (2020). PubMed: 33032611