製品の概要

  • 製品名Anti-Hephaestin antibody
    Hephaestin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Hephaestin
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Hephaestin.

    (Peptide available as ab108244.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in WI38 whole cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab95137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).

ターゲット情報

Anti-Hephaestin antibody 画像

  • Anti-Hephaestin antibody (ab95137) at 1 µg/ml + WI-38 whole cell lysate (ab3960) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 130 kDa
    Observed band size : 130 kDa


    Exposure time : 20 minutes

Anti-Hephaestin antibody (ab95137) 使用論文

ab95137 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Tissue sections (Duodenum)
Specification Duodenum
Permeabilization Yes - 0.1% TritonX in 0.1% PBS
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Aug 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (brain, heart, embryo)
Loading amount 20 µg
Specification brain, heart, embryo
Gel Running Conditions Reduced Denaturing (4-12% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Oct 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"