製品の概要

  • 製品名Anti-Hephaestin antibody
    Hephaestin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Hephaestin
  • アプリケーション適用あり: WB, IHC-Fr, ICC/IF, IHC-FrFlmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 315-425 of Human Hephaestin

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 130 kDa.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/500.
IHC-FrFl Use at an assay dependent concentration.

ターゲット情報

Anti-Hephaestin antibody 画像



  • Predicted band size : 130 kDa
  • ab56729 staining hephaestin (red) in Rat brain capillary endothelial cell tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1.5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 0.3% Triton X-100) for 12 hours at 4°C. A Cy3®-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of hepaestin staining on rat P3 glial culture using ab56729. The cells were fixed with 4% PFA in 0.1M PBS. Mature astrocytes are visible in red. Nucleus stained with Hoechst at high power magnification.

    See Abreview

  • All lanes : Anti-Hephaestin antibody (ab56729) at 1/1000 dilution

    Lane 1 : Whole tissue lysate prepared from murine liver
    Lane 2 : Whole tissue lysate prepared from murine liver

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated sheep anti-mouse polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 130 kDa


    Exposure time : 1 minute

    Image courtesy of Marco Spinazzi by Abreview.

    See Abreview

Anti-Hephaestin antibody (ab56729) 使用論文

This product has been referenced in:
  • McCarthy RC & Kosman DJ Ferroportin and exocytoplasmic ferroxidase activity are required for brain microvascular endothelial cell iron efflux. J Biol Chem 288:17932-40 (2013). WB ; Human . Read more (PubMed: 23640881) »
  • Yang WM  et al. Transient expression of iron transport proteins in the capillary of the developing rat brain. Cell Mol Neurobiol 31:93-9 (2011). IHC-Fr ; Rat . Read more (PubMed: 21061168) »

See all 2 Publications for this product

Product Wall

Thank you for your reply.

Let me know if the protocol suggestions do not improve your results.

Although this order was placed more than 6 months ago, I would be happy to offer you a replacement or refund if we can't get the antibody...

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Thank you for your inquiry. I am sorry to hear you are having difficulty with one of our products.

We have heard that this antibody works in mouse liver in this Abreview. Would you be able to run a control on liver?

http://www.abcam...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Loading amount 20 µg
Specification liver
Gel Running Conditions Reduced Denaturing (4-12% bis-tris)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Marco Spinazzi

Verified customer

投稿 Mar 26 2012

Thank you for your reply.

Your Abreview has successfully been received and one of our colleagues will review it in order to publish your results in our Website.

The Abreview discount code is now activated; you can use it to place a ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Astro-glia)
Specification Astro-glia
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10%
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Mar 21 2012

Thank you for your reply.

If you do not detect the correct band after adjusting your protocol, please let me know and I will either replace or refund the antibody for you.

In the meantime if there is anything else I can help you wit...

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Thank you for your reply.

I do not think that the 65kDa band is Hephaestin, it could be that the antibody is recognizing something non-specifically on skeletal muscle tissue.

However there does seem to be a band at a higher molecula...

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Thank you for contacting Abcam.

You could use http://www.proteinatlas.org/ to check for expression levels in human otherwise you should consult the literature and see what is published about the expression pattern of this protein.

P...

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Thank you for contacting Abcam.

For ab56729, the Anti-Hephaestin antibody from what I can see this protein is detected in breast, colon, bone trabecular cells and fibroblasts. I am not completely familiar with the protein distribution pattern...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Rat Tissue sections (Liver)
Specification Liver
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Dec 01 2011

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