製品の概要

  • 製品名Anti-Hepcidin-25 antibody
    Hepcidin-25 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Hepcidin-25
  • アプリケーション適用あり: ICC/IF, WB, IHC-Fr, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Hepcidin-25.

    (Peptide available as ab31875.)

  • ポジティブ・コントロール
    • HAMP Human Full-length Recombinant Protein (Tagged)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab30760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
WB 1/10 - 1/100. Detects a band of approximately 32 kDa (predicted molecular weight: 3 kDa).

Current batches of ab30760 gave a positive signal in LPS treated serum in WB at a dilution ranging from 1/10 and 1/100. We recommend customers to determine the optimal dilution for their experiments.

IHC-Fr 1/200.
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).
    Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11113131, PubMed:11034317).
  • 組織特異性Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
  • 関連疾患Hemochromatosis 2B
  • 配列類似性Belongs to the hepcidin family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 製品の状態Hepcidin produces a propeptide of 84 amino acids that undergoes enzymatic cleavage into mature peptides of 20, 22, and 25 amino acids. Active peptides are rich in cysteines that form intramolecular bonds and stabilize the beta-sheet structure
  • 別名
    • Hamp antibody
    • HEPC antibody
    • HEPC_HUMAN antibody
    • Hepc20 antibody
    • Hepc25 antibody
    • HEPCIDIN antibody
    • Hepcidin 20 antibody
    • Hepcidin 25 antibody
    • Hepcidin antimicrobial peptide antibody
    • Hepcidin-20 antibody
    • Hepcidin25 antibody
    • HFE2 antibody
    • HFE2B antibody
    • LEAP 1 antibody
    • LEAP-1 antibody
    • LEAP1 antibody
    • Liver expressed antimicrobial peptide antibody
    • Liver-expressed antimicrobial peptide 1 antibody
    • PLTR antibody
    • Putative liver tumor regressor antibody
    see all

Anti-Hepcidin-25 antibody 画像

  • Anti-Hepcidin-25 antibody (ab30760) + LPS treated mouse serum

    Secondary
    Donkey Polyclonal to Anti Rabbit IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 3 kDa

    This image is courtesy of Ruma Raha-Chowdhury (Cambridge University)

  • Hepcidin-25 expression in Rat liver cells using Rabbit polyclonal to Hepcidin-25 (ab30760).Immunohistochemistry was performed on PFA fixed cells, ab30760 was used at 1/1000 incubated overnight at RT. Haemotoxylin is used as a nuclear counterstain in this image.
  • Anti-Hepcidin-25 antibody (ab30760) at 1 µg/ml + HAMP Human Full-length Recombinant Protein (Tagged) at 0.1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 3 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
    ab30760 was tested against Human HAMP Full-length Recombinant Protein (Tagged) predicted to run at 32kDa.
  • ab30760 at a 1/200 dilution staining Hepcidin-25 in human artery tissue sections by Immunohistochemistry (frozen sections) incubated for 24 hours at 20°C. Acetone fixed. Blocked with 0.5% BSA for 10 minutes at 20°C. Secondary used at 7µg/ml Goat anti-rabbit IgG (H+L) conjugated to biotin.
    Immunoperoxydase system and AEC were used to develop the color.

    See Abreview

  • ab30760 staining cultured rat astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab30760 at 1/100 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 24h at 4°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. Hepcidin expression visible perinuclearly,  mainly in the lysosomes.

    See Abreview

Anti-Hepcidin-25 antibody (ab30760) 使用論文

This product has been referenced in:
  • de Seny D  et al. Biomarkers of inflammation and innate immunity in atrophic nonunion fracture. J Transl Med 14:258 (2016). IP . Read more (PubMed: 27599571) »
  • Raha-Chowdhury R  et al. Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci 16:24 (2015). IHC . Read more (PubMed: 25896789) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Liver tissue lysate)
Gel Running Conditions Reduced Denaturing (11% SDS-PAGE)
Loading amount 30 µg
Specification Liver tissue lysate
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 23 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Baboon Tissue sections (Adult liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate, pH 6.0
Permeabilization No
Specification Adult liver
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Formaldehyde
Username

Ms. Marcia Burch

Verified customer

投稿 Mar 22 2016

Thank you for your enquiry.

I am sorry to confirm this antibody is still not available and for the inconvenience. I contacted the laboratories and they confirmed that another batch is currently in production, but due to difficulties with past ...

Read More
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Rat Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 01 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Mouse Recombinant protein (Mouse cell lysate)
Specification Mouse cell lysate
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Type Indirect
Username

Abcam user community

Verified customer

投稿 Oct 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Astrocytes culture)
Specification Astrocytes culture
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Feb 13 2012

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results and for the delay in getting back to you. The details provided will enable us to investigate this ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Sep 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (artery)
Specification artery
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Dec 10 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (rat liver cell lysate)
Loading amount 30 µg
Specification rat liver cell lysate
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Nov 29 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"