Anti-Hepatitis C Virus NS5a 抗体 [388] (ab20342)

製品の概要

  • 製品名Anti-Hepatitis C Virus NS5a antibody [388]
    Hepatitis C Virus NS5a 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [388] to Hepatitis C Virus NS5a
  • 特異性Ab20342 is positive with genotypes 1a and 1b, and is negative with genotype 2a.
  • アプリケーション適用あり: IP, ELISA, ICC/IF, WBmore details
  • 種交差性
    Reacts with HCV. Not yet tested in other species.
  • 免疫原

    Recombinant full length protein (HCV genotype 1a).

  • エピトープEpitope has not been mapped.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)>90% pure
  • ポリ/モノモノクローナル
  • クローン名388
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab20342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration. PubMed: 19839005
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

ターゲット情報

  • 関連性HCV is classified into the genus Hepacivirus of the family Flaviviridae. Like all the members of the family, HCV is an enveloped, single-stranded, positive-sense RNA virus. Its genome (about 9600 nt) is flanked at both termini by conserved, highly structured non-translated regions (NTRs) and encodes a polyprotein precursor (about 3000 aa), which is proteolytically processed by host and viral proteases to produce the structural (core, E1, E2 and p7) and non-structural (NS2, NS3, NS4A, NS4B, NS5A and NS5B) proteins of the virus. Recently, an additional protein has been identified, whose function remains unknown. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008).
  • 別名
    • HCV antibody
    • HCV Non-structural protein 5A antibody
    • Non-structural protein 5A antibody
    • NS5A antibody
    • p56 antibody
    see all

Anti-Hepatitis C Virus NS5a antibody [388] 画像

  • All lanes : Anti-Hepatitis C Virus NS5a antibody [388] (ab20342) at 1/1000 dilution

    Lane 1 : Huh-7 (negative control)
    Lane 2 : HCV Replicon cell line

    Secondary
    Goat anti-mouse IgG conjugated to HRP

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-Hepatitis C Virus NS5a antibody [388] (ab20342) 使用論文

This product has been referenced in:
  • El Abd YS  et al. Neutralizing activities of caprine antibodies towards conserved regions of the HCV envelope glycoprotein E2. Virol J 8:391 (2011). WB ; Hepatitis C Virus . Read more (PubMed: 21819575) »
  • Gonzalez O  et al. The heat shock protein inhibitor Quercetin attenuates hepatitis C virus production. Hepatology 50:1756-64 (2009). IP . Read more (PubMed: 19839005) »

See all 2 Publications for this product

Product Wall

Thanks for sending along your protocol.

1. ab49033 is unlikely to be detected by these antibodies as it only contains about 100 aa of the total protein. As described on datasheet this product containsamino acids"corresponding to amino ac...

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Thank you for contacting us.

This may sound familiar ;-)

This anti-HCVNS4b and NS5aantibodies are mouse monoclonals of IgG2b andIgG2a isotype, respectively.IgG2 antibodies usually comprise 450 amino acids, resulting in a molecular w...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (huh-7 cells replicon)
Specification huh-7 cells replicon
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C
Type Sandwich (Capture)
Username

Abcam user community

Verified customer

投稿 Jul 30 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (liver cell line)
Loading amount 50000 cells
Specification liver cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
Username

Abcam user community

Verified customer

投稿 Jun 28 2006

Thank you for your enquiry. The molecular weight of native HCV NS-5a is a 56kDa. I hope this information helps. Please do not hesitate to contacts us if you need anything further.

Thank you for your enquiry. Ab20342 has not been tested for reactivity to genotype 2a. Also, the epitope has not been determined. Please contact us again if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"