Biotin Anti-Hepatitis C Virus NS4A 抗体 [S4-13] (ab124526)
Key features and details
- Biotin Mouse monoclonal [S4-13] to Hepatitis C Virus NS4A
- Suitable for: Flow Cyt (Intra), WB, ELISA, ICC/IF
- Reacts with: Hepatitis C virus
- Conjugation: Biotin
- Isotype: IgG2b
製品の概要
-
製品名
Biotin Anti-Hepatitis C Virus NS4A antibody [S4-13] -
製品の詳細
Biotin Mouse monoclonal [S4-13] to Hepatitis C Virus NS4A -
由来種
Mouse -
標識
Biotin -
特異性
Specific to Human Hepatitis C Virus NS4A protein of genotype 1b. Not tested in other genotypes -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ELISA, ICC/IFmore details -
種交差性
交差種: Hepatitis C virus -
免疫原
A region of NS4 protein of Hepatitis C Virus genotype 1b expressed in E. coli.
-
エピトープ
N terminal region of the NS4 protein (NS4A). -
ポジティブ・コントロール
- Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
-
特記事項
[biotin] / [IgG] = 11.3
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Proprietary Purification -
特記事項(精製)
Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
S4-13 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124526の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 6 kDa.
|
|
ELISA |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration.
|
特記事項 |
---|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 6 kDa. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
-
関連性
Hepatitis C Virus is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of Hepatitis C Virus with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha -
細胞内局在
Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: Host membrane insertion occurs after processing by the NS3 protease. -
参照データベース
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: P26663 Hepatitis C virus
-
別名
- core protein antibody
- E1 protein antibody
- E2 protein antibody
see all
画像
-
Biotin Anti-Hepatitis C Virus NS4A antibody [S4-13] (ab124526) + Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
Predicted band size: 6 kDa -
ab124526 staining Hepatitis C Virus NS4A in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA. After incubation for 48 hr, the cells were fixed with acetone and Hepatitis C Virus NS4A protein was detected by indirect immunofluorescence staining using this antibody.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab124526 は論文での使用が確認できていません。