製品の概要

  • 製品名Anti-Hepatitis C Virus NS3 antibody [H23]
    Hepatitis C Virus NS3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [H23] to Hepatitis C Virus NS3
  • 特異性Hepatitis C Virus antigen NS3 helicase domain. Reacts very well with JFH-1 strain (genotype 2a)
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 免疫原

    Recombinant full length protein (E. coli).

  • エピトープHelicase domain

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名H23
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab13830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB 1/1000.
IHC-P 1/50.

Tested using paraffin-embedded liver sections from HCV patients.

ターゲット情報

  • 関連性HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • 細胞内局在Endoplasmic reticulum membrane
  • 別名
    • Hepatitis C Virus NS3 antibody
    • NS3 antibody
    • NS3P antibody
    • p70 antibody
    • Serine protease/NTPase/helicase antibody
    see all

Anti-Hepatitis C Virus NS3 antibody [H23] 画像

  • ab13830 used in IHC at 1/50 dilution. Cell line used is Huh7 human hepatoma.
  • The negative control for above picture with Huh7 cells.
  • Image from Kota S et al., PLoS One. 2012;7(2):e32207. Epub 2012 Feb 28.; Fig 3.; doi:10.1371/journal.pone.0032207; February 28, 2012, PLoS ONE 7(2): e32207.

Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830) 使用論文

This product has been referenced in:
  • Li X  et al. Hepatocyte nuclear factor 4a and downstream secreted phospholipase A2 GXIIB regulate production of infectious hepatitis C virus. J Virol 88:612-27 (2014). Read more (PubMed: 24173221) »
  • Germain MA  et al. Elucidating novel hepatitis C virus-host interactions using combined mass spectrometry and functional genomics approaches. Mol Cell Proteomics 13:184-203 (2014). Hepatitis C Virus . Read more (PubMed: 24169621) »

See all 13 Publications for this product

Product Wall

Thank you for your message.

Reviewing the recommended storage details for these antibodies, I can suggest they should not be significantly affected by short term storage for a week or two at +4oC. However, I would recommend to aliquot and fr...

Read More

Thank you for your enquiry. I am sorry it has taken some time to request the immunogen seuqences from our sources and review these for you, thank you for your patience. I can confirm the following mouse Hepatitis C Virus NS3 antibodies have been tes...

Read More

Thank you for sending me the image of your blot. So, you have no bands (just some background) in the NS3 recombinant protein lane, and the same pattern of bands in the other lanes, including the negative control. Just to make sure, do you know that you...

Read More

Thank you for your message and I'm sorry to hear that you are experiencing difficulty with ab13830. It would be helpful if you could send me an image of the blot. You mention no signal but then mention non-specific bands. How strong are these bands? Wh...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"