The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. ab18663 will allow visualization of 0.1 ug/lane of synthetic NS-3 protein.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication.
The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase.
The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
細胞内局在Endoplasmic reticulum membrane
Hepatitis C Virus NS3 antibody
Serine protease/NTPase/helicase antibody
Anti-Hepatitis C Virus NS3 antibody [20-8] (ab18663) 使用論文
has not yet been referenced specifically in any publications.