製品の概要

  • 製品名Anti-Hepatitis C Virus E2 antibody
    Hepatitis C Virus E2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Hepatitis C Virus E2
  • 特異性E2 envelope protein of Hepatitis C Virus.
  • アプリケーション適用あり: ELISA, WB, Conjugationmore details
  • 種交差性
    Reacts with Hepatitis C Virus. Not yet tested in other species.
  • 免疫原

    Recombinant full length E2 (genotype 1a) protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)>95% pure. Sodium sulfate precipitation and ion-exchange chromatography.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab20044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 70 kDa.
Conjugation Use at an assay dependent dilution.

ターゲット情報

  • 関連性Hepatitis C E2 is a virus envelope glycoprotein which forms a heterodimer with the E1 protein. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.
  • 細胞内局在Viral envelope protein.
  • 別名
    • Core protein antibody
    • E1 protein antibody
    • E2 protein antibody
    • Envelope glycoprotein E2 antibody
    • gp68 antibody
    • gp70 antibody
    • HCV E2 antibody
    • NS1 antibody
    • NS2 protein antibody
    • NS3 protease/helicase antibody
    • NS4A protein antibody
    • NS4B protein antibody
    • NS5A protein antibody
    • NS5B RNA dependent RNA polymerase antibody
    • P7 protein antibody
    • Polyprotein antibody
    see all

Anti-Hepatitis C Virus E2 antibody 画像

  • ELISA analysis of Hepatitis C Virus E2 binding to CD81 receptor, using ab20044 to detect Hepatitis C Virus E2 binding.

    A 96-well plate was coated with 100 ng of rho-1D4 antibody. Following overnight incubation at 4°C, the unbound antibody was washed away. The wells were blocked with blocking solution for 2 hours. 100 ng of purified CD81 was added to the wells along with E2 glycoprotein (0.4 to 20 nM) and incubated for 2 hours. ab20044 anti-Hepatitis C Virus E2 antibody (1/2000 dilution) and secondary antibody with alkaline phosphatase conjugate (1/1000 dilution) were added and incubated for 2 hours. Absorbance was measured at 495 nm by a plate-reader.

Anti-Hepatitis C Virus E2 antibody (ab20044) 使用論文

This product has been referenced in:
  • Takayama H  et al. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3:e2314 (2008). ELISA ; Hepatitis C Virus . Read more (PubMed: 18523555) »

See 1 Publication for this product

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ab20044 and ab20852 have not been tested in neutralisation experiment. We have no empirical data to support theuse in the application.

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Following your enquiry I have checked all our HAV, HCV and Parvovirus B19 antibodies and doublechecked with the originators of these if the antibodies would detect intact virions. Thank you for your patience whil...

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Thank you for your enquiry. I can confrim that the immunogen for ab20044 Hepatitis C Virus E2 antibody is a recombinant antigen expressed in CHO cells. This is a polyclonal antibody and therefore recognizes multiple epitopes on the E2 protein, but...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"