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Recombinant full length protein (GST-tag) corresponding to Hepatitis C Virus Core 1b.
Our Abpromise guarantee covers the use of ab2740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Flow Cyt||1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20422251|
|IHC-P||Use at an assay dependent concentration. PubMed: 25155355|
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines.|
ab2740 staining Hepatitis C Virus Core Antigen in the Huh7-Lunet Cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour at 37°C. An undiluted Alexa Fluor® 647-conjugated Goat anti-mouse IgG monoclonal was used as the secondary antibody.
Immunofluorescence analysis of Huh-7.5.1 cells infected with either Hepatitis C virus (J6/JFH-C) or Hepatitis C virus defective in RNA polymerase activity (J6/JFH pol-null).
Hepatitis C Virus Core Antigen (red) was stained with ab2740 at 1/300 dilution, 96 hours after infection. A Cy3®-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody. Nuclei were stained with DAPI (blue).
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