Anti-Hepatitis C Virus Core 1b 抗体 [C7-50] (ab2740)

製品の概要

  • 製品名
    Anti-Hepatitis C Virus Core 1b antibody [C7-50]
  • 製品の詳細
    Mouse monoclonal [C7-50] to Hepatitis C Virus Core 1b
  • 特異性
    Detects hepatitis C virus (HCV) core protein from transfected human and primate cell lines.
  • アプリケーション
    適用あり: IP, Flow Cyt, ICC/IF, IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Hepatitis C virus
  • 免疫原

    Recombinant full length protein (GST-tag) corresponding to Hepatitis C Virus Core 1b.

  • エピトープ
    This antibody recognizes an epitope between amino acid residues 21-40 of HCV core protein. This sequence is conserved among different HCV strains.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 20422251
IHC-P Use at an assay dependent concentration. PubMed: 25155355
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines.

ターゲット情報

  • 関連性
    HCV (Hepatitis C Virus) viral core protein forms the internal viral coat that encapsidates the genomic RNA and is enveloped in a host cell-derived lipid membrane. The hepatitis C virus (HCV) core protein represents the first 177 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. The N terminus of the core protein is involved in transcriptional repression. There are over 20 different subtypes of Hepatitis C Virus; HCV type 1b is mostly found in Europe and Asia. The prevalence of HCV type 1b infection has recently decreased, although it still accounts for most HCV-related cirrhosis and hepatocellular carcinoma. High HCV viremia levels and HCV genotype type 1b are independent predictors for poor response to interferon-alpha therapy. HCV core protein is among the most conserved proteins in HCV and is known to induce sensitization of cytotoxic T lymphocytes (CTL). Therefore, it is a prime candidate for a component of a potential HCV vaccine.
  • 細胞内局在
    Secreted; Host endoplasmic reticulum membrane; Single-pass membrane protein; Host nucleus; Host cytoplasm.
  • 参照データベース
    • 別名
      • Capsid protein C antibody
      • Core protein p19 antibody
      • Core protein p21 antibody
      • Hepatitis C virus core protein antibody

    Anti-Hepatitis C Virus Core 1b antibody [C7-50] 画像

    • ab2740 staining Hepatitis C Virus Core Antigen in the Huh7-Lunet Cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour at 37°C. An undiluted Alexa Fluor® 647-conjugated Goat anti-mouse IgG monoclonal was used as the secondary antibody.

      See Abreview

    • Immunofluorescence analysis of Huh-7.5.1 cells infected with either Hepatitis C virus (J6/JFH-C) or Hepatitis C virus defective in RNA polymerase activity (J6/JFH pol-null).
      Hepatitis C Virus Core Antigen (red) was stained with ab2740 at 1/300 dilution, 96 hours after infection. A Cy3®-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    Anti-Hepatitis C Virus Core 1b antibody [C7-50] (ab2740) 使用論文

    This product has been referenced in:
    • Russelli G  et al. HCV replication in gastrointestinal mucosa: Potential extra-hepatic viral reservoir and possible role in HCV infection recurrence after liver transplantation. PLoS One 12:e0181683 (2017). ICC/IF . Read more (PubMed: 28750044) »
    • Xue J  et al. MEAN inhibits hepatitis C virus replication by interfering with a polypyrimidine tract-binding protein. J Cell Mol Med 20:1255-65 (2016). WB, ICC/IF . Read more (PubMed: 26929148) »

    See all 25 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (human hepatocarcinoma)
    Antigen retrieval step
    None
    Permeabilization
    Yes - H2O2
    Specification
    human hepatocarcinoma
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.05% · Temperature: 24°C
    Fixative
    Methanol
    Username

    Hwanhee Lee

    Verified customer

    投稿 Jul 01 2016

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Human Cell lysate - whole cell (human liver carcinoma cell line)
    Specification
    human liver carcinoma cell line
    Treatment
    transfection of Core protein expressed vector
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 29 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Huh7-Lunet Cells)
    Specification
    Huh7-Lunet Cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 19 2013

    Thank you for confirming the details of this product.

    I am pleased to provide thetesting protocol for ELISA using this antibody which I have copied below. Please note, this is a guideline only and may require some individual optimization. Read More

    It is not known. From epitope mapping it has been shown to be directed against aa 21-40 of the HCV core region. I would suggest seeing how conserved this sequence is in 1a.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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