The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
IHC-P: 1/50. Tested using paraffin-embedded liver sections from HCV patients.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Hepatitis C Virus is the leading cause of liver cancer. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural proteins necessary for viral replication.