Anti-Hepatitis C Virus 抗体 [NS5B-6] (Biotin) (ab122972)

製品の概要

  • 製品名Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin)
    Hepatitis C Virus 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [NS5B-6] to Hepatitis C Virus (Biotin)
  • 標識Biotin
  • 特異性ab122972 recognizes NS5b (also called RNA-directed RNA polymerase).
  • アプリケーション適用あり: WB, ELISA, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Hepatitis C Virus
  • 免疫原

    Recombinant fragment of NS5b protein of Hepatitis C Virus genotype 1b, expressed in E. coli

  • ポジティブ・コントロール
    • Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
  • 特記事項

    Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーConstituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)filter-sterilized
  • ポリ/モノモノクローナル
  • クローン名NS5B-6
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 70 kDa).
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
  • 細胞内局在Virus surface
  • 参照データベース
    • 別名
      • Genome polyprotein antibody
      • HCV antibody
      • Hepatitis C virus polyprotein antibody

    Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) 画像

    • Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) (ab122972) + Lysate of chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA

      Predicted band size : 70 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    • ab122972, staining Hepatitis C Virus NS5b protein in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by immunofluorescence.

    Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) (ab122972) 使用論文

    This product has been referenced in:
    • Manabe S  et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). Read more (PubMed: 8291245) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"