Biotin Anti-Hepatitis C Virus 抗体 [NS5B-6] (ab122972)
Key features and details
- Biotin Mouse monoclonal [NS5B-6] to Hepatitis C Virus
- Suitable for: Flow Cyt (Intra), WB, ELISA, ICC/IF
- Reacts with: Hepatitis C virus
- Conjugation: Biotin
- Isotype: IgG2b
製品の概要
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製品名
Biotin Anti-Hepatitis C Virus antibody [NS5B-6]
Hepatitis C Virus 一次抗体 製品一覧 -
製品の詳細
Biotin Mouse monoclonal [NS5B-6] to Hepatitis C Virus -
由来種
Mouse -
標識
Biotin -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ELISA, ICC/IFmore details -
種交差性
交差種: Hepatitis C virus -
免疫原
Recombinant fragment of NS5b protein of Hepatitis C Virus genotype 1b, expressed in E. coli
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ポジティブ・コントロール
- Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
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特記事項
Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
特記事項(精製)
filter-sterilized -
ポリ/モノ
モノクローナル -
クローン名
NS5B-6 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122972の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 70 kDa).
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ELISA |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 70 kDa). |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. -
細胞内局在
Virus surface -
参照データベース
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: P27958 Hepatitis C virus
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別名
- Genome polyprotein antibody
- HCV antibody
- Hepatitis C virus polyprotein antibody
画像
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Biotin Anti-Hepatitis C Virus antibody [NS5B-6] (ab122972) + Lysate of chimp liver cells infected with recombinant
vaccinia virus containing a HCV genome cDNA
Predicted band size: 70 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
NS5b protein is detected as a 58kDa band. -
ab122972, staining Hepatitis C Virus NS5b protein in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by immunofluorescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab122972 は 3 報の論文で使用されています。
- Zhang MQ et al. A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells. Autophagy 16:2167-2179 (2020). PubMed: 31996071
- Zhang MQ et al. Differential Effects of Autophagy-Related 10 Protein on HCV Replication and Autophagy Flux Are Mediated by Its Cysteine44 and Cysteine135. Front Immunol 9:2176 (2018). PubMed: 30319633
- Manabe S et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). PubMed: 8291245