Anti-Hepatitis B Virus X antigen 抗体 [X36C] (ab2741)


  • 製品名
    Anti-Hepatitis B Virus X antigen antibody [X36C]
    Hepatitis B Virus X antigen 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [X36C] to Hepatitis B Virus X antigen
  • 由来種
  • アプリケーション
    適用あり: IHC-P, ELISA, IP, WBmore details
  • 種交差性
    交差種: Hepatitis B virus
  • 免疫原

    Recombinant full length protein corresponding to Hepatitis B Virus X antigen.


  • 製品の状態
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度
  • 一次抗体 備考
    Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein-protein interaction.
  • ポリ/モノ
  • クローン名
  • アイソタイプ
  • 研究分野


Our Abpromise guarantee covers the use of ab2741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml.


  • 関連性
    Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC.
  • 参照データベース
    • 別名
      • HBV X antigen antibody
      • HBx antibody
      • HBxAg antibody


    • All lanes : Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741) at 1/2000 dilution

      Lane 1 : MIHA hepatocytes
      Lane 2 : MIHA hepatocytes infected with lentivirus containing EGFP alone
      Lane 3 : MIHA hepatocytes infected with lentivirus containing full length Hepatitis B Virus X antigen
      Lanes 4-5 : MIHA hepatocytes infected with lentivirus containing truncated Hepatitis B Virus X antigen


    This product has been referenced in:
    • Huang P  et al. Hepatitis B Virus X Protein (HBx) Is Responsible for Resistance to Targeted Therapies in Hepatocellular Carcinoma: Ex Vivo Culture Evidence. Clin Cancer Res 21:4420-30 (2015). Read more (PubMed: 26059188) »
    • Yip WK  et al. Carboxyl-terminal truncated HBx regulates a distinct microRNA transcription program in hepatocellular carcinoma development. PLoS One 6:e22888 (2011). WB . Read more (PubMed: 21829663) »

    See all 2 Publications for this product

    レビューと Q&A

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    Unfortunately laboratory has confirmed that they will not be able to provide the positive control. I am sorry I am not able to help further with this inquiry.

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    I have checked into whether anything is know about the epitope of the anti-Hepatitis B Virus X antigen antibodies ab235, ab2741 and ab39716. I can o...

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    Thank you for contacting us and your interest in our products.

    The request for a quote for the antibodies mentioned has been passed on to one of my colleagues who will get back to you shortly with this information. In regards to your other qu...

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    Thank you for your message. Unfortuantley, I am unable to obtain precise information about which subtypes of Hepatitis B virus these antibodies detect. We aim to provide as much information as possible to customers, and I am sorry this is not possib...

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    The immunogen used was the whole recombinant Hepatitis B X protein and the epitope has not been determined.