The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Direct FA staining: Use at an assay dependent dilution. A starting range of 1:10-1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.