Anti-Hepatitis B Virus pre S2 Antigen 抗体 [S26] (ab8635)
Key features and details
- Mouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen
- Suitable for: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WB
- Reacts with: Hepatitis B virus
- Isotype: IgG1
製品の概要
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製品名
Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] -
製品の詳細
Mouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen -
由来種
Mouse -
特異性
This antibody reacts with HBV Large and Middle Surface Antigen but not small forms (both glycosylated and non glycosylated species). -
アプリケーション
適用あり: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WBmore details -
種交差性
交差種: Hepatitis B virus -
免疫原
Tissue, cells or virus corresponding to Hepatitis B virus Hepatitis B Virus pre S2 Antigen. Purified serum HBV surface antigen
Database link: A0FDJ9 -
エピトープ
aa positions 131-139 (adw nomenclature) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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ポリ/モノ
モノクローナル -
クローン名
S26 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8635の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
IHC-FoFr |
1/100.
|
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IHC-Fr |
1/100.
|
|
Immunomicroscopy |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
|
IHC-P |
1/100.
|
|
WB | (2) |
1/1000.
|
特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-FoFr
1/100. |
IHC-Fr
1/100. |
Immunomicroscopy
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
1/100. |
WB
1/1000. |
ターゲット情報
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関連性
The human hepatitis B virus (HBV) is a DNA virus that causes chronic infections that frequently lead to the development of cirrhosis and cellular hepatocellular carcinoma. The HBsAg (surface antigen) gene is one long open reading frame but contains three in frame "start" (ATG) codons that divide the gene into three sections, pre-S1, pre-S2, and S. Because of the multiple start codons, polypeptides of three different sizes called large, middle, and small (pre-S1 + pre-S2 + S, pre-S2 + S, or S) are produced. The preS2 domain is the minimal functional unit of transcription activators that are encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC). Pre-S2 is a diagnostically important surface antigen of human hepatitis B virus. The PreS domain (S1 + S2) of Hepatitis B virus (HBV) surface antigen may be a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes. -
細胞内局在
Virion membrane (By similarity). -
参照データベース
- Entrez Gene: 2828293 Hepatitis B virus
- SwissProt: A0FDJ9 Hepatitis B virus
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別名
- HBV pre S2 Antigen antibody
- HBV pre S2 protein antibody
- Hepatitis B Virus pre S2 protein antibody
see all
画像
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All lanes : Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from HEK cells
Lanes 2-3 : Whole cell lysate prepared from HEK cells transfected with pCI M
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Sheep anti-mouse HRP conjugated polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 33,36,39 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab8635 は 5 報の論文で使用されています。
- Ma A et al. A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B. Hum Vaccin Immunother 16:756-778 (2020). PubMed: 31687879
- Li YX et al. Hepatitis B Virus Middle Protein Enhances IL-6 Production via p38 MAPK/NF-?B Pathways in an ER Stress-Dependent Manner. PLoS One 11:e0159089 (2016). PubMed: 27434097
- Draz MS et al. Hybrid nanocluster plasmonic resonator for immunological detection of hepatitis B virus. ACS Nano 6:7634-43 (2012). PubMed: 22934963
- Villet S et al. In vitro characterization of viral fitness of therapy-resistant hepatitis B variants. Gastroenterology 136:168-176.e2 (2009). ICC/IF . PubMed: 18996386
- Glebe D et al. Pre-s1 antigen-dependent infection of Tupaia hepatocyte cultures with human hepatitis B virus. J Virol 77:9511-21 (2003). PubMed: 12915565