Anti-Heparan Sulfate Proteoglycan 2 抗体 [A74] (ab23418)

製品の概要

  • 製品名
    Anti-Heparan Sulfate Proteoglycan 2 antibody [A74]
    Heparan Sulfate Proteoglycan 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [A74] to Heparan Sulfate Proteoglycan 2
  • 特異性
    ab23418 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WB, IP, IHC-Fr, ELISAmore details
  • 種交差性
    交差種: Cow, Human
  • 免疫原

    Corneal endothelial cells (Cow).

  • エピトープ
    Epitope is located in domain V of Perlecan.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 22278369
ICC/IF Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 469 kDa.
IP Use at an assay dependent concentration.
IHC-Fr 1/100.
ELISA 1/2000.
AP Use at an assay dependent concentration. Can be used to separate recombinant domain I from full length perlecan.

ターゲット情報

  • 機能
    Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
    Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
    The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity.
  • 組織特異性
    Found in the basement membranes.
  • 関連疾患
    Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
    Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.
  • 配列類似性
    Contains 4 EGF-like domains.
    Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 11 laminin EGF-like domains.
    Contains 3 laminin G-like domains.
    Contains 3 laminin IV type A domains.
    Contains 4 LDL-receptor class A domains.
    Contains 1 SEA domain.
  • 翻訳後修飾
    Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
    N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Basement membrane specific heparan sulfate proteoglycan core protein antibody
    • Endorepellin (domain V region) antibody
    • Heparan sulfate proteoglycan of basement membrane antibody
    • HSPG 2 antibody
    • HSPG antibody
    • Hspg2 antibody
    • LG3 peptide antibody
    • Perlecan antibody
    • PGBM_HUMAN antibody
    • PLC antibody
    • Schwartz Jampel syndrome 1 (chondrodystrophic myotonia) antibody
    • SJA antibody
    • SJS antibody
    • SJS1 antibody
    see all

画像

  • Immunohistochemical analysis of Human ovary tissue, staining Heparan Sulfate Proteoglycan 2 with ab23418.

    Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/10 in diluent) for 30 minutes. An HRP-conjugated anti-mouse IgG was used as the secondary antibody.

    See Abreview

  • ab23418 staining Heparan Sulfate Proteoglycan 2 in Human fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with methanol, permeabilized with 1% Triton X-100 and blocked with 5% BSA for 20 minutes at 22°C. Samples were incubated with primary antibody (1/600 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated goat anti-mouse monoclonal (1/200) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Ganesan MK  et al. Three-Dimensional Coculture Model to Analyze the Cross Talk Between Endothelial and Smooth Muscle Cells. Tissue Eng Part C Methods 23:38-49 (2017). Rat, Human . Read more (PubMed: 27923320) »
  • Puerta-Guardo H  et al. Dengue Virus NS1 Disrupts the Endothelial Glycocalyx, Leading to Hyperpermeability. PLoS Pathog 12:e1005738 (2016). Read more (PubMed: 27416066) »

See all 7 Publications for this product

レビューと Q&A

Thank you once again for your enquiries. I was in touch with our source for these antibodies again today, and I am pleased I now have the information for you.   1. Can ab26265 and ab23418 be used to detect for all kind of Heparan sulfate or are they...

Read More
Application
Flow Cytometry
Sample
Rhesus monkey Cell (chorioretinal endothelial cell)
Permeabilization
No
Gating Strategy
size and granularity
Specification
chorioretinal endothelial cell
Preparation
Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with Accutase
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation
Paraformaldehyde
Username

Andrea Cabrera

Verified customer

投稿 Sep 22 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human decelluarized adipose matrix)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate buffer pH 6.0 for 40 minutes at 97.0 ±1 °C
Permeabilization
No
Specification
human decelluarized adipose matrix
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 29 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.05% typsin
Sample
Human Tissue sections (human whole fat tissue)
Specification
human whole fat tissue
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 05 2015

The epitope of ab23418 is located in domain V of Perlecan, which is from C-terminal amino acids 3663-4391 according to the uniprot file below:

http://www.uniprot.org/uniprot/P98160

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human fibroblast)
Specification
Human fibroblast
Fixative
Methanol
Permeabilization
Yes - triton X-100 1%
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 28 2012

Thank you for calling Abcam earlier today.
I am sorry that you experienced issues with ab23418 in western blot. As we discussed I am sending you ab82346 as a free of charge replacement. Your new order number is *********
.
Please let me ...

Read More

Thank you very much for contacting us and for your patience. I apologise for the delay.

We have contacted the originator of the Anti-Heparan Sulfate Proteoglycan 2 antibody [A74]ab23418and enquired about epitope details. Unfortunately, there ...

Read More

Code de remise pour ab86750 : ******
Code de remise pour ab23418 : ******
Date d’expiration pour ces deux codes : JJ MM AAAA
Je suis ravi que vous souhaitiez participer à notre offre et tester ab86750 et ab23418 en ICC/IF. Les codes de r...

Read More

Thank you for your inquiry. Unfortunately we have no information on any aa sequences for this antibody other than it is corneal endothelial cells from cow. Below is listed the following prices: Please let me know if I can be of furt...

Read More

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録