Rabbit polyclonal to HEPACAM2
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog
Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (NYSCLVRNPV SEMESDIIMP IIYYGPYGLQ VNSDKGLKVG EVFTVDLGEA) of Human HEPACAM2 (NP_001034461).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Membrane. Golgi apparatus. Cytoplasm > cytoskeleton > spindle. In interphase, localized in the Golgi apparatus. During mitosis, associated with mitotic splindles and centrosomes.
Information by UniProt
- AI987662 antibody
- HECA2_HUMAN antibody
- HEPACAM 2 antibody
Western blot - Anti-HEPACAM2 antibody (ab82977)
Anti-HEPACAM2 antibody (ab82977) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
51 kDaObserved band size:
60 kDa (why is the actual band size different from the predicted?
)Additional bands at:
45 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
has not yet been referenced specifically in any publications.
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