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RabMAb

Anti-Hemopexin 抗体 [EPR5610] (ab124935)

製品の概要

  • 製品名
    Anti-Hemopexin antibody [EPR5610]
    Hemopexin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5610] to Hemopexin
  • アプリケーション
    適用あり: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • ポジティブ・コントロール
    • WB: Human fetal liver and plasma lysates. IHC-P: Human liver tissue. Flow Cyt: HepG2 cells.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/200.
WB 1/10000 - 1/50000. Detects a band of approximately 70-75 kDa (predicted molecular weight: 52 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

ターゲット情報

  • 機能
    Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • 組織特異性
    Expressed by the liver and secreted in plasma.
  • 配列類似性
    Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.
  • 翻訳後修飾
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta 1B glycoprotein antibody
    • Beta-1B-glycoprotein antibody
    • FLJ56652 antibody
    • HEMO antibody
    • HEMO_HUMAN antibody
    • Hemopexin antibody
    • Hpx antibody
    • HX antibody
    see all

Anti-Hemopexin antibody [EPR5610] 画像

  • All lanes : Anti-Hemopexin antibody [EPR5610] (ab124935) at 1/10000 dilution

    Lane 1 : Fetal liver lysate
    Lane 2 : Human plasma lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size : 52 kDa
    Observed band size : 70-75 kDa (why is the actual band size different from the predicted?)
  • ab124935 staining Hemopexin in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Flow cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Hemopexin (red) with ab124935 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • ab124935, at 1/250 dilution, staining Hemopexin in paraffin-embedded Human liver tissue by Immunohistochemistry.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-Hemopexin antibody [EPR5610] (ab124935) 使用論文

ab124935 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample
Human Tissue sections (human liver)
Specification
human liver
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Fixative
Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

投稿 Mar 12 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Tissue lysate - whole (hu liver, hu colon, hu colon carcinoma & bovine li)
Specification
hu liver, hu colon, hu colon carcinoma & bovine li
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Steffen Rickelt

Verified customer

投稿 Jan 08 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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