Anti-Hemopexin 抗体 (ab116532)
Key features and details
- Rabbit polyclonal to Hemopexin
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Hemopexin antibody
Hemopexin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hemopexin -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Rabbit, Human, Chimpanzee, Macaque monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in both Mouse and Rat Sciatic Nerve tissue lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab116532の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 52 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 52 kDa). |
ターゲット情報
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機能
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. -
組織特異性
Expressed by the liver and secreted in plasma. -
配列類似性
Belongs to the hemopexin family.
Contains 5 hemopexin-like domains. -
翻訳後修飾
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3263 Human
- Entrez Gene: 15458 Mouse
- Entrez Gene: 100174097 Orangutan
- Entrez Gene: 58917 Rat
- Omim: 142290 Human
- SwissProt: P02790 Human
- SwissProt: Q91X72 Mouse
- SwissProt: Q5R543 Orangutan
see all -
別名
- Beta 1B glycoprotein antibody
- Beta-1B-glycoprotein antibody
- FLJ56652 antibody
see all
画像
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All lanes : Anti-Hemopexin antibody (ab116532) at 1 µg/ml
Lane 1 : Mouse Sciatic Nerve Tissue Lysate
Lane 2 : Rat Sciatic Nerve Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Hemopexin has been shown to migrate at ~70 kDa under reducing conditions by Madore et al PMID: 9930744. We welcome customer feedback. Hemopexin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116532 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab116532 は論文での使用が確認できていません。