Anti-Hemoglobin subunit alpha 抗体 [EP3607] (ab109444)

製品の概要

  • 製品名Anti-Hemoglobin subunit alpha antibody [EP3607]
    Hemoglobin subunit alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP3607] to Hemoglobin subunit alpha
  • 特異性Mouse and rat tissues tested (lung and kidney) gave negative or low sensitivity staining in IHC-P. This antibody may not be suitable for IHC with mouse or rat samples. If you have any questions, please contact our Scientific Support team.
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Hemoglobin subunit alpha.

  • ポジティブ・コントロール
    • WB: K562,fetal liver, and fetal heart lysates IHC-P: Human spleen and Human colon tissues
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EP3607
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab109444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能Involved in oxygen transport from the lung to the various peripheral tissues.
    • 組織特異性Red blood cells.
    • 関連疾患Heinz body anemias
      Alpha-thalassemia
      Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
      Hemoglobin H disease
    • 配列類似性Belongs to the globin family.
    • 翻訳後修飾The initiator Met is not cleaved in variant Thionville and is acetylated.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alpha 1 globin antibody
      • Alpha globin antibody
      • Alpha one globin antibody
      • Alpha-globin antibody
      • HBA_HUMAN antibody
      • HBA1 antibody
      • HBA2 antibody
      • Hemoglobin alpha 1 antibody
      • Hemoglobin alpha 1 chain antibody
      • Hemoglobin alpha 1 globin chain antibody
      • Hemoglobin alpha 2 antibody
      • Hemoglobin alpha chain antibody
      • Hemoglobin subunit alpha antibody
      • MGC126895 antibody
      • MGC126897 antibody
      see all

    Anti-Hemoglobin subunit alpha antibody [EP3607] 画像

    • All lanes : Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : Fetal heart lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 15 kDa
      Observed band size : 14 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded Human spleen tissue, using ab109444 at a 1/250 dilution.
    • Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded Human colon tissue, using ab109444 at a 1/250 dilution.

    Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444) 使用論文

    ab109444 has not yet been referenced specifically in any publications.

    Product Wall

    Vielen Dank für Ihren Anruf.

    Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456und so...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Thank you for your reply and for testing the antibodies.

    Unfortunately, we do not have another lot in stock for ab109444 - just the same lot. Please let me know if one of the other Anti-Hemoglobin subunit alpha antibodies we carry, ab92492 o...

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    According to our records, ab6721 or ab109444 was proving difficult to use in WBand we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaiting more details in order to help us better ...

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    Thank you for contacting us.
    I am sorry to hear that one of these antibodies is notworking properly.

    As we discussed over the phone, trying a no-primary control (with secondary antibody only) would be another way to check if the problem...

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    Thank you for the reply.

    The recommended dilutions on our datasheets provide a concentration to begin with, however the optimal dilution does have to be determined by the end user. Therefore I strongly recommend that you try various concentra...

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    Thank you for sending that protocol information.

    If I have read the information correctly, then there are a few protocol variations that may work:

    1 - Did the customer only try a 1/5000 primary antibody dilution? If so, I strongly s...

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    I am sorry that the replacement antibody did not prove to succesful. Unfortunately the protocol that you attached did not come through. Could you resend the protocol so that I can look it over and see if there are any suggestions that I can make.
    ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"