Anti-Hemoglobin subunit alpha 抗体 (ab82871)
Key features and details
- Rabbit polyclonal to Hemoglobin subunit alpha
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Hemoglobin subunit alpha antibody
Hemoglobin subunit alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hemoglobin subunit alpha -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab92492.
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アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Chimpanzee, Monkey, Baboon, Macaque monkey, Rhesus monkey, Gorilla, Dolphin, Orangutan -
免疫原
Synthetic peptide corresponding to Human Hemoglobin subunit alpha aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab93083) -
ポジティブ・コントロール
- This antibody gave a positive signal in human bone marrow and spleen tissue lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab82871の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/250. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
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特記事項 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/250. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). |
ターゲット情報
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機能
Involved in oxygen transport from the lung to the various peripheral tissues. -
組織特異性
Red blood cells. -
関連疾患
Heinz body anemias
Alpha-thalassemia
Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
Hemoglobin H disease -
配列類似性
Belongs to the globin family. -
翻訳後修飾
The initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
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参照データベース
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Omim: 141800 Human
- Omim: 141850 Human
- SwissProt: P69905 Human
- SwissProt: P01946 Rat
- Unigene: 449630 Human
- Unigene: 654744 Human
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別名
- Alpha 1 globin antibody
- Alpha globin antibody
- Alpha one globin antibody
see all
画像
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All lanes : Anti-Hemoglobin subunit alpha antibody (ab82871) at 1/250 dilution
Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
IHC image of Hemoglobin A1c staining in human bone marrow formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82871, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ab17976 staining Hemoglobin A1c in Rat Lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA in PBS for 20 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hours at 25°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab82871 は 1 報の論文で使用されています。
- Præstmark KA et al. Skin blood perfusion and cellular response to insertion of insulin pen needles with different diameters. J Diabetes Sci Technol 8:752-9 (2014). PubMed: 24876418