製品の概要
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製品名Anti-Hemoglobin antibody (HRP)
Hemoglobin 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to Hemoglobin (HRP)
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標識HRP
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特異性The antibody has been tested in ELISA and IEP with a Human Hemaglobin Calibrator/Standard but has yet to be tested against endogenous protein.
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アプリケーション適用あり: ICC, ELISA, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Rabbit, Dog, Chimpanzee, Rhesus monkey
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免疫原
Human Hemoglobin
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ポジティブ･コントロール
- recombinant human hemoglobin
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特記事項
Molar enzyme/antibody protein ratio is 4:1.
法規制情報
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C.
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バッファーPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.1M Sodium chloride, 50mM HEPES. pH 7.1 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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アプリケーション
Our Abpromise guarantee covers the use of ab19362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | ||
| ELISA | ||
| WB |
追加情報
ELISA: 1/10000 - 1/100000.
ICC: 1/200 - 1/500.
WB: 1/1000 - 1/10000.
Colorimetric detection: 1/1000 - 1/10000
Chemiluminescent detection: 1/1000 - 1/30000
Predicted molecular weight: 16 kDa (the protein is a tetramer; each subunit has a molecular weight of 16kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ICC: 1/200 - 1/500.
WB: 1/1000 - 1/10000.
Colorimetric detection: 1/1000 - 1/10000
Chemiluminescent detection: 1/1000 - 1/30000
Predicted molecular weight: 16 kDa (the protein is a tetramer; each subunit has a molecular weight of 16kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能Involved in oxygen transport from the lung to the various peripheral tissues.
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組織特異性Red blood cells.
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関連疾患Defects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders. -
配列類似性Belongs to the globin family.
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翻訳後修飾The initiator Met is not cleaved in variant Thionville and is acetylated.
- Information by UniProt
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参照データベース
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Entrez Gene: 3043 Human
- Entrez Gene: 3047 Human
- Entrez Gene: 693930 Rhesus monkey
- Omim: 141800 Human
- Omim: 141900 Human
- Omim: 142200 Human
see all -
別名
- 3-prime alpha-globin gene antibody
- Alpha 2 globin chain antibody
- Alpha globin antibody
see all
Anti-Hemoglobin antibody (HRP) 画像
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Western blotAnti-Hemoglobin antibody (HRP) (ab19362) at 1/1000 dilution +Natural Human Hemoglobin protein (ab77858) at 0.01 µg
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
Anti-Hemoglobin antibody (HRP) (ab19362) 使用論文
ab19362 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"