Anti-Hemoglobin 抗体 [901] (ab20079)


  • 製品名Anti-Hemoglobin antibody [901]
    Hemoglobin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [901] to Hemoglobin
  • 特異性Adult human hemoglobin. It has not been tested for reactivity to fetal hemoglobin.
  • アプリケーション適用あり: ELISA, Flow Cytmore details
  • 種交差性
    交差種: Human
    非交差種: Sheep, Horse, Chicken, Cow, Pig
  • 免疫原

    Full length native protein (purified) (Human).



Our Abpromise guarantee covers the use of ab20079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Use at an assay dependent dilution to detect and quantitate human Hemoglobin.

Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 機能Involved in oxygen transport from the lung to the various peripheral tissues.
  • 組織特異性Red blood cells.
  • 関連疾患Defects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
    Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
    Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
  • 配列類似性Belongs to the globin family.
  • 翻訳後修飾The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3-prime alpha-globin gene antibody
    • Alpha 2 globin chain antibody
    • Alpha globin antibody
    • alpha one globin antibody
    • alpha-1 globin antibody
    • Alpha-globin antibody
    • Beta globin antibody
    • CD113t C antibody
    • CD31 antibody
    • Delta globin antibody
    • Erythremia, beta-globin type, included antibody
    • Gamma 1 globin antibody
    • Hb F Agamma antibody
    • HBA 1 antibody
    • HBA 2 antibody
    • HBA antibody
    • HBA T3 antibody
    • HBA-T2 antibody
    • HBA_HUMAN antibody
    • HBA1 antibody
    • HBA2 antibody
    • HBB antibody
    • Hbb-y antibody
    • HBD antibody
    • Hbe1 antibody
    • HBG 1 antibody
    • HBG antibody
    • HBG1 antibody
    • HBGA antibody
    • HBGR antibody
    • HBH antibody
    • Hemoglobin alpha 1 antibody
    • hemoglobin alpha 1 globin chain antibody
    • Hemoglobin alpha chain antibody
    • Hemoglobin alpha locus antibody
    • Hemoglobin alpha locus 1 antibody
    • hemoglobin alpha-1 chain antibody
    • Hemoglobin beta antibody
    • Hemoglobin beta chain antibody
    • Hemoglobin beta chain complex antibody
    • Hemoglobin beta locus antibody
    • Hemoglobin gamma 1 chain antibody
    • Hemoglobin gamma A antibody
    • Hemoglobin gamma A chain antibody
    • Hemoglobin gamma antibody
    • Hemoglobin subunit alpha antibody
    • Hemoglobin subunit beta antibody
    • Hemoglobin subunit gamma 1 antibody
    • hemoglobin, gamma, regulator of antibody
    • Hemoglobin--gamma locus, 136 alanaine antibody
    • HSGGL1 antibody
    • LVV-hemorphin-7 antibody
    • Methemoglobinemia, beta-globin type, included antibody
    • MGC126895 antibody
    • MGC126897 antibody
    • Minor alpha-globin locus antibody
    • PRO2979 antibody
    see all

Anti-Hemoglobin antibody [901] 画像

  • Overlay histogram showing K562 cells stained with ab20079 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20079, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Hemoglobin antibody [901] (ab20079) 使用論文

ab20079 has not yet been referenced specifically in any publications.

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Thank you for your patience in waiting for a response regarding ab401 and ab402. The source of these antibodies returned my email today and stated that in all likelihood antibodies ab401 and ab402 to human Hemoglobin do not recognize fetal Hb. They ha...

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