製品の概要

  • 製品名Anti-Hemogen antibody
    Hemogen 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Hemogen
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Hemogen.

    (Peptide available as ab66549.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Lysates: Testis Tissue, Ovary Tissue, Pancreas Tissue, Lymph node Tissue, Brain Tissue (data not shown), Spleen Tissue (data not shown), Thymus Tissue (data not shown), HeLa Whole Cell (data not shown), SW480 Whole Cell (data not shown), HepG2 Whole Cell (data not shown)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 55 kDa).

ターゲット情報

  • 機能Regulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).
  • 組織特異性Expressed in hematopoietic precursor cells (at protein level). Detected in CD34+ and K562 leukemia cell line (at protein level). Expressed in bone marrow, testis, thymus. Also expressed in thymus and thyroid tumors, non-Hodgkin lymphoma, various leukemia cell lines, peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) of patients with leukemia.
  • 発生段階Expressed in fetal liver, kidney and brain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EDAG antibody
    • EDAG-1 antibody
    • Erythroid differentiation-associated gene protein antibody
    • Hemgn antibody
    • HEMGN_HUMAN antibody
    • Hemogen antibody
    • Hemopoietic gene protein antibody
    • Negative differentiation regulator protein antibody
    see all

Anti-Hemogen antibody 画像

  • All lanes : Anti-Hemogen antibody (ab66550) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)
    Lane 3 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 4 : Human lymph node tissue lysate - total protein (ab29871)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

Anti-Hemogen antibody (ab66550) 使用論文

ab66550 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab66550.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"