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Our Abpromise guarantee covers the use of ab13248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 4 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa).|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ELISA||Use a concentration of 2 µg/ml.|
|IP||Use at an assay dependent concentration. PubMed: 24098580|
|Sandwich ELISA||Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Heme Oxygenase 1 (ab13243). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Heme Oxygenase 1 (ab13243) as Detection.|
|IHC-P||Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
Hek293 & HL60 presumed negative or very low expression.
Loading control GAPDH at 38kDa
IHC image of Hem Oxygensae 1 staining in a section of formalin fixed, paraffin embedded normal human spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13248, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab13248 at 10µg/ml staining Heme Oxygenase 1 in human lung cancer A2 cells by flow cytometery. The left image repersents staining with isotype control antibody and the right image show staining with ab13248.
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