Anti-Heme Oxygenase 1 抗体 [HO-1-1] (ab13248)


  • 製品名Anti-Heme Oxygenase 1 antibody [HO-1-1]
    Heme Oxygenase 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HO-1-1] to Heme Oxygenase 1
  • アプリケーション適用あり: WB, ICC, Flow Cyt, ELISA, IP, Sandwich ELISA, IHC-P, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Dog, Human, Monkey
    交差が予測される動物種: Pig
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 1-30 of Human Heme Oxygenase 1.

  • ポジティブ・コントロール
    • Recombinant Human or Rat HO-1 (Hsp32) Protein. HEK293 treated with 30 uM hemin for 18hrs (see Abreview).



Our Abpromise guarantee covers the use of ab13248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 4 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa).
ICC 1/500.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA Use a concentration of 2 µg/ml.
IP Use at an assay dependent concentration. PubMed: 24098580
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Heme Oxygenase 1 (ab13243). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Heme Oxygenase 1 (ab13243) as Detection.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/300.


Anti-Heme Oxygenase 1 antibody [HO-1-1] 画像

  • Predicted band size : 34.6 kDa
    The following proteins and lysates were electrophoresed; Lane 1 - Heme-Oxygenase-1 (Hsp32) Protein (50ng), lane 2 - Heme-Oxygenase-2 protein NSP-550 (100ng), lane 3 - MDBK Cell Lysate (20ug), lane 4 - Mouse liver microsome (20ug) and lane 5 - Dog liver microsome (20ug). ab13248 was applied at a concentration of 4ugml-1.
  • Standard Curve for Heme Oxygenase 1 (Analyte: Heme Oxygenase 1 protein (Tagged) (ab85243)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) at 5µg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1 (ab13243) at 0.5µg/ml.
  • Lane 1 : MW marker
    Lanes 2 - 7 : Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

    Lane 1 : As above
    Lane 2 : Recombinant Rat Heme Oxygenase 1
    Lane 3 : Recombinant Human Heme Oxygenase 1
    Lane 4 : Recombinant Human Heme Oxygenase 2
    Lane 5 : MDBK cell lysate
    Lane 6 : Dog liver microsome
    Lane 7 : Mouse liver microsome

    Predicted band size : 34.6 kDa
  • Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1/250 dilution + Human microsome lysate

    Predicted band size : 34.6 kDa
  • ab13248 staining Heme Oxygenase 1 in murine heart tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized, blocked using 2% serum for 1 hour at 30°C and then incubated with ab13248 at a 1/300 dilution for 12 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat polyclonal used at a 1/500 dilution.

    The green colour indicate Heme Oxygenase 1.
    The blue colour indicate nucleus stain DAPI.

    See Abreview

  • Immunohistochemistry analysis of human spleen tissue stained with ab13248 detecting Heme Ozygenase 1 at 10µg/ml

  • ab13248 at 10µg/ml staining Heme Oxygenase 1 in human lung cancer A2 cells by flow cytometery. The left image repersents staining with isotype control antibody and the right image show staining with ab13248.


Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) 使用論文

This product has been referenced in:
  • Chao MW  et al. N-acetylcysteine attenuates lipopolysaccharide-induced impairment in lamination of Ctip2-and Tbr1- expressing cortical neurons in the developing rat fetal brain. Sci Rep 6:32373 (2016). WB . Read more (PubMed: 27577752) »
  • Shearer JJ  et al. Inorganic Arsenic-Related Changes in the Stromal Tumor Microenvironment in a Prostate Cancer Cell-Conditioned Media Model. Environ Health Perspect 124:1009-15 (2016). WB ; Human . Read more (PubMed: 26588813) »

See all 35 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative Acetone

Abcam user community

Verified customer

投稿 Jul 14 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Rat Cell lysate - whole cell (RAT AORTIC SMOOTH MUSCLE CELLS)
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. P Bingi

Verified customer

投稿 Oct 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma primary cells)
Loading amount 30 µg
Specification Glioblastoma primary cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Apr 16 2013

Thank you for contacting us.

I have checked the details you have kindly provided. I would suggest the following troubleshooting steps;

- Please add cocktail of protease inhibitors in lysates.
- This antibody works perfect in reduc...

Read More

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the ph...

Read More

Thank you for your email.

We have another kit, the Protein Quantitation Kit (Bradford Assay), ab102535, which may also be of interest to you. Or we also have Bradford reagent available from our catalogue, ab119216 and ab119216 for 500 and 1000...

Read More

Thank you for contacting Abcam regarding ab13248.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check th...

Read More

Thank you for your reply.

Both the rabbit monoclonal ab52947 and the ab13243 have been shown to work well in IHC of paraffin embedded tissue sections. The results achieved can be seenfrom the images presentedon the datasheet of these products...

Read More

I hope your grant application is going well. We have several antibodies against the targets you have mentioned which may be of interest to you:


I would suggest looking at the rabbit polyclonal

Read More

Thank you for sending us the additional information. This is very helpful.
I think your protocol looks fine. I'd like to ask a couple more questions, if you don't mind:
1) Are you using the same sample preparation protocol for all your sample...

Read More