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RabMAb

Anti-Heme Oxygenase 1 抗体 [EPR1390Y] (ab68477)

製品の概要

  • 製品名
    Anti-Heme Oxygenase 1 antibody [EPR1390Y]
    Heme Oxygenase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1390Y] to Heme Oxygenase 1
  • アプリケーション
    適用あり: ICC/IF, WB, IP, Flow Cytmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    within Human Heme Oxygenase 1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P09601

  • ポジティブ・コントロール
    • HepG2, A549, rat kidney, rat spleen, mouse kidney cell lysate.
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP 1/20.
Flow Cyt Use at an assay dependent concentration.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
    • 配列類似性
      Belongs to the heme oxygenase family.
    • 細胞内局在
      Microsome. Endoplasmic reticulum.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 32 kD antibody
      • bK286B10 antibody
      • D8Wsu38e antibody
      • heat shock protein 32 kD antibody
      • heat shock protein 32kD antibody
      • Heat shock protein antibody
      • Heme oxygenase (decycling) 1 antibody
      • Heme oxygenase 1 antibody
      • Hemox antibody
      • HMOX 1 antibody
      • Hmox antibody
      • Hmox1 antibody
      • HMOX1_HUMAN antibody
      • HO 1 antibody
      • HO antibody
      • HO-1 antibody
      • HO1 antibody
      • Hsp32 antibody
      see all

    Anti-Heme Oxygenase 1 antibody [EPR1390Y] 画像

    • All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/1000 dilution

      Lane 1 : Hek293
      Lane 2 : HL60
      Lane 3 : HeLa
      Lane 4 : A549
      Lane 5 : Hu spleen
      Lane 6 : Ms spleen
      Lane 7 : Rt spleen

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye® 800CW Goat anti Rabbit

      Predicted band size : 33 kDa

      Hek293 & HL60 presumed negative or very low expression.

      Loading control GAPDH at 38kDa

    • ab68477 (unpurified) staining Heme Oxygenase 1 in mouse embryonic fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1.5% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/100 in 1X HBSS + 0.02% Triton X-100 + 1.5% FBS) for 3 hours at 25°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Heme Oxygenase 1 with purified ab68477 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Negative control 1: PBS only.

    • Flow cytometry analysis of A549 (human lung carcinoma) cells labeling with purified ab68477 at 1/200 dilution ( 1ug/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) )(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    • ab68477 (purified) at 1/20 immunoprecipitating Heme Oxygenase 1 in A549 (Human lung carcinoma cell line) whole cell lysate.

      Lane 1 (input): A549 whole cell lysate (10ug).

      Lane 2 (+): ab68477 + A549 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133267 in HeLa whole cell lysate.

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/20000 dilution (purified)

      Lane 1 : Rat kidney lysate
      Lane 2 : Rat spleen lysate
      Lane 3 : Mouse kidney lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 33 kDa
      Observed band size : 33 kDa

      Blocking and dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/20000 dilution (purified)

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 33 kDa
      Observed band size : 33 kDa

      Blocking and dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/2000 dilution (purified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP conjugated Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 33 kDa
      Observed band size : 33 kDa
      Additional bands at : 125-130 kDa,30 kDa,48 kDa,52 kDa. We are unsure as to the identity of these extra bands.

    Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) 使用論文

    This product has been referenced in:
    • Li J  et al. Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model. Stem Cell Res Ther 8:107 (2017). WB ; Rat . Read more (PubMed: 28482861) »
    • Shen C  et al. Resveratrol pretreatment attenuates injury and promotes proliferation of neural stem cells following oxygen-glucose deprivation/reoxygenation by upregulating the expression of Nrf2, HO-1 and NQO1 in vitro. Mol Med Rep 14:3646-54 (2016). WB ; Rat . Read more (PubMed: 27573874) »

    See all 6 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 May 13 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (rat intestinal epithelial cells (RIE-1))
    Permeabilization
    Yes - 0.2% TritonX-100
    Specification
    rat intestinal epithelial cells (RIE-1)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Feb 12 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Chinese Hamster Cell (Chinese hamster ovary cell)
    Permeabilization
    Yes - 0.2% TritonX-100
    Specification
    Chinese hamster ovary cell
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Feb 12 2016

    Application
    Western blot
    Sample
    Spodoptera frugiperda (SF9 cells) Cell lysate - whole cell (sf9 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    100000 cells
    Treatment
    30 nM Bortezomib for 16 hours
    Specification
    sf9 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

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    Verified customer

    投稿 Feb 09 2016

    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (rat intestinal epithelial cells (RIE-1))
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    100000 cells
    Treatment
    100 nM Bortezomib for 16 hours
    Specification
    rat intestinal epithelial cells (RIE-1)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 09 2016

    Application
    Western blot
    Sample
    Chinese Hamster Cell lysate - whole cell (Chinese hamster ovary cell)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    100000 cells
    Treatment
    100 nM Bortezomib for 16 hours
    Specification
    Chinese hamster ovary cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 09 2016

    Application
    Flow Cytometry
    Sample
    Mouse Cell (Mouse Lewis Lung Carcinoma cells)
    Permeabilization
    Yes - 0.2% Triton X-100
    Gating Strategy
    No gating
    Specification
    Mouse Lewis Lung Carcinoma cells
    Preparation
    Cell harvesting/tissue preparation method: trypsin
    Sample buffer: Hanks’ Balanced Salt solution (HBSS)
    Fixation
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jan 22 2016

    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Transplanted lung tumor section)
    Specification
    Transplanted lung tumor section
    Username

    Abcam user community

    Verified customer

    投稿 Dec 21 2015

    Application
    IHC - Wholemount
    Sample
    Human Tissue (Breast invasive ductal carcinoma)
    Specification
    Breast invasive ductal carcinoma
    Username

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    Verified customer

    投稿 Dec 21 2015

    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (COS-7 cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    100000 cells
    Treatment
    30 nM Bortezomib for 24 hours
    Specification
    COS-7 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Username

    Abcam user community

    Verified customer

    投稿 Jun 22 2015

    1-10 of 16 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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