製品の概要

  • 製品名Anti-Heme Oxygenase 1 antibody [EP1391Y]
    Heme Oxygenase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1391Y] to Heme Oxygenase 1
  • アプリケーション適用あり: WB, IP, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Guinea pig, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of human Heme Oxygenase 1

  • ポジティブ・コントロール
    • Fetal liver lysate, human liver tissue and HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

アプリケーション

Our Abpromise guarantee covers the use of ab52947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IP 1/60.
Flow Cyt 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
  • 配列類似性Belongs to the heme oxygenase family.
  • 細胞内局在Microsome. Endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 32 kD antibody
    • bK286B10 antibody
    • D8Wsu38e antibody
    • heat shock protein 32 kD antibody
    • heat shock protein 32kD antibody
    • Heat shock protein antibody
    • Heme oxygenase (decycling) 1 antibody
    • Heme oxygenase 1 antibody
    • Hemox antibody
    • HMOX 1 antibody
    • Hmox antibody
    • Hmox1 antibody
    • HMOX1_HUMAN antibody
    • HO 1 antibody
    • HO antibody
    • HO-1 antibody
    • HO1 antibody
    • Hsp32 antibody
    see all

Anti-Heme Oxygenase 1 antibody [EP1391Y] 画像

  • Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947) at 1/2000 dilution + fetal liver lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • Overlay histogram showing HEK293 cells stained with ab52947 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52947, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • ab52947 at 1/100-1/250 dilution staining Heme Oxygenase 1 in human liver by Immunohistochemistry, Paraffin embedded tissue.

Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947) 使用論文

This product has been referenced in:
  • Zhang J  et al. Comparisons of ethanol extracts of chinese propolis (poplar type) and poplar gums based on the antioxidant activities and molecular mechanism. Evid Based Complement Alternat Med 2015:307594 (2015). Mouse . Read more (PubMed: 25802536) »
  • Cinti A  et al. Simultaneous Overexpression of Functional Human HO-1, E5NT and ENTPD1 Protects Murine Fibroblasts against TNF-a-Induced Injury In Vitro. PLoS One 10:e0141933 (2015). WB, ICC/IF ; Mouse . Read more (PubMed: 26513260) »

See all 9 Publications for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (RAW 264.7)
Specification RAW 264.7
Treatment Control No treatment, 5mM N-ACETYL CYSTEINE, 50uM buthionine sulphoximine
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Vamsi Kodali

Verified customer

投稿 Jun 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Guinea pig Tissue lysate - whole (liver and brain)
Specification liver and brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (endothelial cells)
Loading amount 10000 cells
Specification endothelial cells
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Mar 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"