製品の概要

  • 製品名Anti-Heme Oxygenase 1 antibody
    Heme Oxygenase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Heme Oxygenase 1
  • アプリケーション適用あり: WB, IHC-Fr, ICC/IF, Sandwich ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Dog, Human
  • 免疫原

    Recombinant rat HO-1 (Hsp32) lacking the membrane spanning region

  • ポジティブ・コントロール
    • Recombinant Human and Rat HO-1 (Hsp32) Protein. IHC-P: FFPE mouse spleen normal and rat spleen normal.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab13243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa).
IHC-Fr 1/200.
ICC/IF Use a concentration of 1 - 5 µg/ml.
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) as Capture.
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
  • 配列類似性Belongs to the heme oxygenase family.
  • 細胞内局在Microsome. Endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 32 kD antibody
    • bK286B10 antibody
    • D8Wsu38e antibody
    • heat shock protein 32 kD antibody
    • heat shock protein 32kD antibody
    • Heat shock protein antibody
    • Heme oxygenase (decycling) 1 antibody
    • Heme oxygenase 1 antibody
    • Hemox antibody
    • HMOX 1 antibody
    • Hmox antibody
    • Hmox1 antibody
    • HMOX1_HUMAN antibody
    • HO 1 antibody
    • HO antibody
    • HO-1 antibody
    • HO1 antibody
    • Hsp32 antibody
    see all

Anti-Heme Oxygenase 1 antibody 画像

  • All lanes : Anti-Heme Oxygenase 1 antibody (ab13243) at 1/1000 dilution

    Lane 1 : Hek293
    Lane 2 : HL60
    Lane 3 : HeLa
    Lane 4 : A549
    Lane 5 : Hu spleen
    Lane 6 : Ms spleen
    Lane 7 : Rt spleen

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye® 800CW Goat anti Rabbit

    Predicted band size : 34.6 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    Hek293 & HL60 presumed negative or very low expression.

    Loading control GAPDH at 38kDa

  • IHC image of Heme Oxygenase 1 staining in formalin fixed, paraffin embedded rat spleen normal tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13243, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Heme Oxygenase 1 antibody (ab13243) (Rat microsomes)

    Predicted band size : 34.6 kDa
  • Alzheimer diseased section stained with ab13243.
  • ICC/IF image of ab13243 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13243, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Standard Curve for Heme Oxygenase 1 (Analyte: Heme Oxygenase 1 protein (Tagged) (ab85243)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) at 5µg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1 (ab13243) at 0.5µg/ml.

プロトコール

Anti-Heme Oxygenase 1 antibody (ab13243) 使用論文

This product has been referenced in:
  • Guo C  et al. Protocatechualdehyde Protects Against Cerebral Ischemia-Reperfusion-Induced Oxidative Injury Via Protein Kinase Ce/Nrf2/HO-1 Pathway. Mol Neurobiol 54:833-845 (2017). Read more (PubMed: 26780453) »
  • de Araújo IW  et al. Analgesic and anti-inflammatory actions on bradykinin route of a polysulfated fraction from alga Ulva lactuca. Int J Biol Macromol 92:820-830 (2016). IHC . Read more (PubMed: 27481342) »

See all 23 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Dec 29 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Normal rat brain)
Specification Normal rat brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 10 2013

Vielen Dank für Ihre Anfrage.

Ich habe vier primäre AK für Sie herausgesucht, die alle in einer relativ hohen Verdünnung eingesetzt werden können, so dass sie für eine Weile reichen sollten. Das Sie alle Proben nu...

Read More

Thank you for your reply.

I will then let the order through as it stands. We will endeavor to have the product with you as soon as possible but as I am sure you can understand we are reliant on delivery companies to move the package on our beh...

Read More

Thank you for your reply.

We are indeed currently running an offer with our Cell Imaging book if you purchase any secondary antibody. Unfortunately I am unable to extend this offer to primary antibodies. I have had a look at the book and it i...

Read More

I hope your grant application is going well. We have several antibodies against the targets you have mentioned which may be of interest to you:

HO-1

I would suggest looking at the rabbit polyclonal http://www.abcam.com/Heme-Oxygenas...

Read More

Thank you very much for your reply.

We are sending a free of charge vial of ab79854 on the order ***, which should arrive tomorrow. Please keep me updated about the results with this replacement, and let me know if there is anything else that...

Read More

Thank you very much for your reply and for sending this additional information.

Would you be interested in tryingab79854or ab13248 free of charge? I can send either of these, if you would like. Alternatively I can issue a credit or refund. Read More

Thank you again for your call earlier this week and for your patience while I have been in touch with the lab.

During the QC testing of this antibody, only a band at the expected molecular weight was seen in lysate samples.30ug of lysate was ...

Read More

Thank you so much for sending the images.

I will forward the lab's protocol and comments as soon as I have heard back from them. Please let me know if you need anything else in the meantime.

1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"