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Recombinant rat HO-1 (Hsp32) lacking the membrane spanning region
Our Abpromise guarantee covers the use of ab13243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa).|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|Sandwich ELISA||Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) as Capture.|
|IHC-P||Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Hek293 & HL60 presumed negative or very low expression.
Loading control GAPDH at 38kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Heme Oxygenase 1 antibody (ab13243)
IHC image of Heme Oxygenase 1 staining in formalin fixed, paraffin embedded rat spleen normal tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13243, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.