Anti-Hematopoiesis related Macrophage antibody [ER-HR3]
Rat monoclonal [ER-HR3] to Hematopoiesis related Macrophage
ab15636 recognises the majority of blood monocytes and a subset of mature resident macrophages, especially those located in hemopoietic organs. Does not cross react with other leukocytes.
The antigen is found on up to 70% of circulating monocytes; all other leukocytes are ab15636 negative. It is also found on a subpopulation (about 30%) of bone marrow cells, mainly consisting of myeloid cells.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/25 - 1/50.
IHC-P: Use at a concentration of 10 µg/ml (1:100).
IHC-Fr (acetone): Use at a concentration of 10 µg/ml (1:100).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Macrophage are any of the many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, circulating for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content and become macrophages. The morphology of macrophages varies among different tissues and between normal and pathologic states, and not all macrophages can be identified by morphology alone. However, most macrophages are large cells with a round or indented nucleus, a well developed Golgi apparatus, abundant endocytotic vacuoles, lysosomes, and phagolysosomes, and a plasma membrane covered with ruffles or microvilli. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes (lysozyme, collagenases, elastase, acid hydrolases), several complement components and coagulation factors, some prostaglandins and leukotrienes, and several regulatory molecules (interferon, interleukin 1). Among the cells now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans type and foreign body type multinucleated giant cells (in inflamed tissues).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hematopoiesis related Macrophage antibody [ER-HR3] (ab15636)This image is a courtesy of Anonymous Abreview
ab15636 staining Hematopoiesis related Macrophage in mouse spleen tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval with Tris HCl 0.1M pH 9.0 and then blocking with 5% BSA for 30 minutes at RT was performed. The primary antibody was diluted 1/100 and incubated with sample for 1 hour in PBS + 1% BSA. A Biotin conjugated goat polyclonal to rat IgG was used at dilution 1/500 as secondary antibody.
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