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Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab20084 as a replacement.
Our Abpromise guarantee covers the use of ab24779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 63 kDa.|
|Flow Cyt||Use at an assay dependent concentration.|
Immunofluorescence staining of HeLa cells transfected without (A) or with (B, C) multiple tag plasmid using ab24779 (1:1000 dilution). An Alexa Fluor® 594 Goat anti-mouse IgG was used as the secondary antibody (1:250). Nuclei stained with DAPI (blue). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope.