製品の概要

  • 製品名Anti-HELZ antibody
    HELZ 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to HELZ
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human, Recombinant Fragment
    交差が予測される動物種: Mouse, Cow
  • 免疫原

    Recombinant fragment: MEDRRAEKSC EQACESLKRQ DYEMALKHCT EALLSLGQYS MADFTGPCPL EIERIKIESL LYRIASFLQL KNYVQADEDC RHVLGEGLAK GEDAFRAVLC , corresponding to amino acids 1-101 of Human HELZ, NP_055692, with a 26kDa tag.

  • ポジティブ・コントロール
    • HeLa cells and HeLa cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab77645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75, 240 kDa (predicted molecular weight: 219 kDa).
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged HELZ is approximately 0.03ng/ml as a capture antibody.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 関連性HELZ (Helicase with zinc finger) belongs to the superfamily I class of RNA helicases. Alteration of RNA secondary structure may subsequently influence interactions with proteins or other nucleic acids.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • DKFZp586G1924 antibody
    • Down regulated in human cancers antibody
    • DRHC antibody
    • Helicase with zinc finger antibody
    • HUMORF 5 antibody
    • HUMORF5 antibody
    • KIAA0054 antibody
    • MGC163454 antibody
    • Probable helicase with zinc finger domain antibody
    see all

Anti-HELZ antibody 画像

  • Anti-HELZ antibody (ab77645) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 219 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-HELZ antibody (ab77645) at 5 µg/ml + HeLa cell lysate (nuclear) at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 219 kDa
    Observed band size : 219 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
  • ab77645 at 10µg/ml staining HELZ in Hela cells.
  • Detection limit for this product is ~0.03ng/ml as a capture antibody.

Anti-HELZ antibody (ab77645) 使用論文

ab77645 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77645.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"